Gentamicin is an important member of the aminoglycoside class of antibiotics having broader spectrum with extend to ototoxicity and nephrotoxcity. A reliable and accurate high performance liquid chromatography (HPLC) procedure was established using Agilent Zorbax Eclipse plus phenyl hexyl C18 (4.6 × 250 mm, 5 μm) column and UV detection at 330 nm. Recovery of gentamicin from biological and pharmaceutical samples was more than 95-98% after spiking the drug. The sensitivity of method is predicted by calculating a lower level of the limit of detection and lower limit of quantification (0.286 and 0.867 μg/mL, respectively. The procedure was used to quantify gentamicin in biological fluids and pharmaceuticals dosage form.

庆大霉素是氨基糖苷类抗生素的重要成员，具有更广的谱，可扩展至耳毒性和肾毒性。使用 Agilent Zorbax Eclipse plus 苯基己基 C18（4.6 × 250 mm，5 μm）色谱柱和 330 nm 紫外检测建立了可靠且准确的高效液相色谱 (HPLC) 程序。加标药物后，生物和药物样品中庆大霉素的回收率超过 95-98%。通过计算检测下限和定量下限（分别为 0.286 和 0.867 μg/mL）来预测方法的灵敏度。该程序用于定量生物体液和药物剂型中的庆大霉素。