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Mesoporous nanosilica loaded with doxycycline to enhance antimicrobial property and mechanical strength at hybrid layer
International Journal of Adhesion and Adhesives ( IF 3.4 ) Pub Date : 2021-08-18 , DOI: 10.1016/j.ijadhadh.2021.102975
Nawaf Labban 1 , Hanan N. Al-Otaibi 1 , Abdulaziz Binrayes 1 , Abdullah Saleh Aljamhan 2 , Afnan F. Alfouzan 1 , Sara Mohammad Al Taweel 1 , Mansour K. Assery 3
Affiliation  

Aim

This study aimed to formulate and characterize the drug-release, physicochemical, and biological properties of doxycycline (DOX)-loaded mesoporous nanosilica (MNS) within experimental resin primers and to investigate the resin-dentin interfacial structure, properties, and performance in terms of microtensile bond strength (uTBS) under different clinically simulating biodegradation/aging conditions using ex-vivo teeth models for a duration of 12 months.

Methodology

MNS was formulated utilizing sol-gel method and loaded with DOX. The morphological, physicochemical, and spectral characterization of the nanocarriers was performed along with drug-release kinetics at varying pH. After incorporating DOX@MNS using two varying concentrations (0.25 wt% and 0.5 wt%) in dentin bonding agent, the modified adhesive was studied for drug-release kinetics at varying pH (7.4, 6.5 and 5.2), degree of conversion (DC), antimicrobial MTT assay, and cytotoxicity. Sectioned teeth were bonded using DOX@MNS modified bonding agent and studied for μTBS at immediate and 12 months storage time in artificial saliva.

Results

DOX-loaded MNS showed a considerable pH-dependent drug release behavior. The greatest percentage of DOX release was seen with 0.5 wt% DOX-loaded MNS at pH 5.2. A 0.5 wt% DOX-loaded MNS-modified adhesive samples demonstrated more profound antibacterial potential against Streptococcus mutans. After 12 months in artificial saliva, the 0.25 wt% DOX-loaded MSN-modified adhesive exhibited the highest μTBS.

Conclusion

The incorporation of 0.5 wt% DOX-loaded MNS in dentin bonding agent yielded superior antibacterial and enhanced bond strength for dentin bonding.



中文翻译:

载有强力霉素的介孔纳米二氧化硅可增强混合层的抗菌性能和机械强度

目的

本研究旨在配制和表征载有强力霉素 (DOX) 的介孔纳米二氧化硅 (MNS) 在实验树脂底漆中的药物释放、理化和生物学特性,并研究树脂-牙本质界面结构、性质和性能使用离体牙齿模型在不同的临床模拟生物降解/老化条件下进行为期 12 个月的微拉伸粘合强度 (uTBS) 。

方法

MNS 采用溶胶-凝胶法配制并加载 DOX。纳米载体的形态、物理化学和光谱表征与不同 pH 下的药物释放动力学一起进行。在牙本质粘合剂中加入两种不同浓度(0.25 wt%和 0.5 wt%)的DOX@MNS 后,研究了改性粘合剂在不同 pH(7.4、6.5 和 5.2)、转化度(DC)下的药物释放动力学、抗菌 MTT 测定和细胞毒性。切片牙齿使用 DOX@MNS 改性粘合剂粘合,并在人工唾液中立即和 12 个月的储存时间研究 μTBS。

结果

负载 DOX 的 MNS 显示出相当大的 pH 依赖性药物释放行为。在 pH 5.2 下,0.5 wt% DOX 负载 MNS 的 DOX 释放百分比最大。0.5 wt% 的DOX 负载 MNS 改性粘合剂样品显示出对变形链球菌更强大的抗菌潜力。在人工唾液中 12 个月后,0.25 wt% DOX 负载的 MSN 改性粘合剂表现出最高的 μTBS。

结论

在牙本质粘接剂中加入 0.5 wt% DOX 负载的 MNS 产生了优异的抗菌性和增强的牙本质粘接的粘接强度。

更新日期:2021-08-25
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