当前位置: X-MOL 学术Fungal Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Can colonization by an endophytic fungus transform a plant into a challenging host for insect herbivores?
Fungal Biology ( IF 2.5 ) Pub Date : 2021-08-18 , DOI: 10.1016/j.funbio.2021.08.001
Charbel Al Khoury 1
Affiliation  

Endophytic growth of arthropod pathogenic fungi can parasitize insect herbivores without causing damage to the crop. However, studies addressing this tritrophic interaction are absent. Here, the endophytic arthropod pathogenic fungus Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordyciptaceae), the polyphagous two-spotted spider mite Tetranychus urticae Koch (Trombidiformes: Tetranychidae), and its preferred plant host Phaseolus vulgaris L. (Fabales: Fabaceae) were selected to study the multi-kingdom interactions among plants, arthropods, and entomopathogenic fungi. Real-Time PCR analysis of nine defense-related genes revealed that a broad range of plant defense mechanisms is activated in response to the endophytic growth of B. bassiana. Moreover, we studied the molecular mechanism adapted by the two-spotted spider mite that underlies resistance. The analysis of 41 detoxification genes revealed that relatively moderate, high, and few numbers of genes were changed in the adults, nymphs, and eggs stages of T. urticae, respectively, after inoculation on colonized tissues of P. vulgaris. The endophytic growth of B. bassiana can have a negative effect on the growth and performance of the pest, in a developmental stage-dependent manner, by priming plant defense pathways. In parallel, the herbivore induces a broad range of detoxification genes that could potentially be involved in adaptation to endophytically colonized plant tissues.



中文翻译:

内生真菌的定殖能否将植物转变为昆虫食草动物的具有挑战性的宿主?

节肢动物病原真菌的内生生长可以寄生于食草昆虫而不会对作物造成损害。然而,缺乏解决这种三营养相互作用的研究。在这里,内生节肢动物病原真菌白僵菌(Balsamo) Vuillemin (Hypocreales: Cordyciptaceae)、多食性二斑叶螨Tetranychus urticae Koch (Trombidiformes: Tetranychidae)及其首选植物宿主Phaseolus vulgaris L. (Fabales: Fabaceae)选择研究植物、节肢动物和昆虫病原真菌之间的多界相互作用。九个防御相关基因的实时 PCR 分析表明,广泛的植物防御机制响应于内生生长而被激活。B. 巴西亚娜。此外,我们研究了抵抗力的二斑叶螨适应的分子机制。对41个解毒基因的分析表明,在荨麻疹定殖组织接种后,荨麻疹成虫期、若虫期和卵期的基因分别发生了相对中度、高度和少数的基因变化B. bassiana的内生生长可以通过启动植物防御途径,以发育阶段依赖的方式对害虫的生长和性能产生负面影响。同时,食草动物诱导了广泛的解毒基因,这些基因可能参与适应内生定植的植物组织。

更新日期:2021-08-18
down
wechat
bug