Abstract

More than 70% of heat resistance enzymes, such as lipase, cutinase, cellulase, Alpha amylase, L-aspartas, are being used in industries which are producing by thermophillic microorganisms. The purpose of this research is Isolation, molecular identification of thermophillic bacteria from the soil of hotest area in Iran and measuring extracellular enzymes activities and kinetics. According to the sampling principles, taking sample from the soil is done from the 5 cm depth of the soil and it became isolated and identified by using ribotyping stub. According to standard methods, the enzyme activities of isolated stub were measured and analyzed. Licheniformis was identified in in ribotyping of the isolated sample. This bacterium had produced the highest amount of cellulase which was 17.5 u/ml, after 72 hours, while pH was equal to 8. As well, the highest amount of protease production was done under alkaline conditions. The asparaginase and lipase activity levels in the isolated Bacillus licheniformis are 4 and 19 enzyme units per ml respectively at 55 °C, while pH is equal to 8. Bacillus licheniformis strain extracted from Gandome Beryan area is an appropriate source of heat-resistant extracellular enzymes with suitable enzyme activity level which can be used in various industries.

摘要

超过 70% 的耐热酶，如脂肪酶、角质酶、纤维素酶、α 淀粉酶、L-天冬氨酸酶，被用于生产嗜热微生物的行业。本研究的目的是从伊朗最热地区的土壤中分离、分子鉴定嗜热细菌，并测量细胞外酶的活性和动力学。根据采样原则，从土壤中5cm深度取样，并通过核糖体分型存根进行分离和鉴定。按照标准方法，对分离出的残株的酶活性进行测定和分析。在分离样品的核糖体分型中鉴定了地衣菌。这种细菌在 72 小时后产生的纤维素酶量最高，为 17.5 u/ml，而 pH 值等于 8。在碱性条件下产生最高量的蛋白酶。分离出的天冬酰胺酶和脂肪酶活性水平地衣芽孢杆菌在55°C下分别为每毫升4和19个酶单位，而pH值等于8。从Gandome Beryan地区提取的地衣芽孢杆菌菌株是具有合适酶活性水平的耐热胞外酶的合适来源，可以使用在各个行业。