N-Hydroxysuccinimide esters of small molecules are widely used to modify biomolecules such as antibodies or proteins. Primary amine groups preferably react with the ester to form covalent amide bonds. Currently, protocols strongly recommend replacing the buffer reagent tris(hydroxymethyl)aminomethane, and it has even been proposed as a stop reagent. Here, we show that TRIS indeed does not interfere with biotinylation of biomolecules with NHS chemistry.

中文翻译：

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三（羟甲基）氨基甲烷与 N-羟基琥珀酰亚胺酯化学的相容性：TRIS 缓冲液中肽和蛋白质的生物素化

小分子的N-羟基琥珀酰亚胺酯被广泛用于修饰生物分子，如抗体或蛋白质。伯胺基团优选与酯反应形成共价酰胺键。目前，协议强烈建议更换缓冲试剂三（羟甲基）氨基甲烷，甚至有人建议将其作为终止试剂。在这里，我们表明 TRIS 确实不会干扰具有 NHS 化学的生物分子的生物素化。