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TomoAlign: A novel approach to correcting sample motion and 3D CTF in CryoET
Journal of Structural Biology ( IF 3 ) Pub Date : 2021-08-18 , DOI: 10.1016/j.jsb.2021.107778
Jose-Jesus Fernandez 1 , Sam Li 2
Affiliation  

TomoAlign is a software package that integrates tools to mitigate two important resolution limiting factors in cryoET, namely the beam-induced sample motion and the contrast transfer function (CTF) of the microscope. The package is especially focused on cryoET of thick specimens where fiducial markers are required for accurate tilt-series alignment and sample motion estimation. TomoAlign models the beam-induced sample motion undergone during the tilt-series acquisition. The motion models are used to produce motion-corrected subtilt-series centered on the particles of interest. In addition, the defocus of each particle at each tilt image is determined and can be corrected, resulting in motion-corrected and CTF-corrected subtilt-series from which the subtomograms can be computed. Alternatively, the CTF information can be passed on so that CTF correction can be carried out entirely within external packages like Relion. TomoAlign serves as a versatile tool that can streamline the cryoET workflow from initial alignment of tilt-series to final subtomogram averaging during in situ structure determination.



中文翻译:

TomoAlign:一种在 CryoET 中校正样本运动和 3D CTF 的新方法

TomoAlign 是一个软件包,它集成了一些工具来减轻 cryoET 中两个重要的分辨率限制因素,即光束引起的样品运动和显微镜的对比度传递函数 (CTF)。该软件包特别专注于厚样品的低温 ET,其中需要基准标记以进行准确的倾斜系列对齐和样品运动估计。TomoAlign 模拟在倾斜系列采集期间经历的光束引起的样本运动。运动模型用于产生以感兴趣的粒子为中心的运动校正的 subtilt 系列。此外,每个倾斜图像上每个粒子的散焦被确定并可以被校正,从而产生运动校正和 CTF 校正的 subtilt-series,从中可以计算 subtomograms。或者,可以传递 CTF 信息,以便可以完全在 Relion 等外部包中进行 CTF 校正。TomoAlign 作为一种多功能工具,可以简化从倾斜系列的初始对齐到最终亚断层图平均的 cryoET 工作流程原位结构测定。

更新日期:2021-09-01
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