There is a worldwide effort to increase the efficiency of potato (Solanum tuberosum L.) cultivar development by using inbred diploid breeding lines. This activity is impeded by the cost and effort required to produce dihaploids from cultivated tetraploid potatoes. We developed a high throughput dihaploid production method based on the 60-year-old method of Peloquin and Hougas. Red Norland inflorescences from commercial fields were transferred to greenhouses. As buds developed, pollen from the dihaploid inducer IVP 101 was applied systematically to thousands of stigmas per trial. Berries were harvested 21 days after pollination. Seeds of putative dihaploids lacking a seed spot marker were retained and ploidy was confirmed using flow cytometry. We recovered 23 dihaploids from 21,651 pollinations. This is a promising method for systematically carrying out thousands of pollinations since the cost of field-grown flowers is dramatically less than that of greenhouse-grown flowers.

全世界都在努力提高马铃薯的效率（Solanum tuberosumL.) 使用近交二倍体育种系开发品种。从栽培四倍体马铃薯生产二单倍体所需的成本和努力阻碍了这项活动。我们开发了一种基于 Peloquin 和 Hougas 已有 60 年历史的方法的高通量二单倍体生产方法。来自商业田地的红色诺兰花序被转移到温室中。随着芽的发育，每次试验将来自二单倍体诱导剂 IVP 101 的花粉系统地应用于数千个柱头。授粉后21天收获浆果。保留缺乏种子斑点标记的推定二单倍体的种子，并使用流式细胞术确认倍性。我们从 21,651 次授粉中回收了 23 个二单倍体。