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High-performance thin-layer chromatography based approach for bioassay and ATR–FTIR spectroscopy for the evaluation of antioxidant compounds from Asparagus racemosus Willd. aerial parts
Biomedical Chromatography ( IF 1.8 ) Pub Date : 2021-08-18 , DOI: 10.1002/bmc.5230
Gouhar Jahan Ashraf 1 , Priya Das 1 , Tarun Kumar Dua 1 , Paramita Paul 1 , Gouranga Nandi 1 , Ranabir Sahu 1
Affiliation  

Asparagus racemosus Willd. is widely used to combat various diseases owing to its medicinal properties. In this study, arial parts of A. racemosus were investigated for their total phenolic content, total flavonoid content and antioxidative potential. A high-performance thin-layer chromatography (HPTLC) method combined with effect-directed-analysis was also developed to screen the antioxidant effects of A. racemosus and quantify biologically active compounds on chromatograms from A. racemosus. Total phenolics (154 mg gallic acid equivalent/g), flavonoid contents (497 mg quercetin/g) and IC50 (15.25 μg/ml) were found to be higher in methanolic extract of A. racemosus than in n-hexane, chloroform and ethyl acetate extracts. HPTLC hyphenated with chemical derivatizations (DPPH•, p-anisaldehyde/sulfuric acid, and ferric chloride) was used to evaluate antioxidant activity and the presence of phytosterols, terpenoids and polyphenolic contents. The same compounds at 100*retention factor = 58, 68, 74 and 65 in extracts were responsible for antioxidant activity. Hyphenated HPTLC allowed a rapid characterization of the active compound with a combination of effect-directed-analysis and attenuated total reflectance–Fourier transform infrared spectroscopy. Spectral analysis of the band from attenuated total reflectance identified myricetin, quercetin, p-coumaric acid and caffeic acid as responsible for the antioxidant activity.

中文翻译:

基于高性能薄层色谱法的生物测定和 ATR-FTIR 光谱法用于评估来自 Asparagus racemosus Willd 的抗氧化化合物。空中零件

总状芦笋Willd。由于其药用特性,被广泛用于对抗各种疾病。在这项研究中,调查了总状花序的总酚含量、总黄酮含量和抗氧化潜力。还开发了一种结合效果导向分析的高效薄层色谱 (HPTLC) 方法,用于筛选 A. racemosus 的抗氧化作用并量化A. racemosus色谱图中的生物活性化合物。总酚(154 mg 没食子酸当量/g)、类黄酮含量(497 mg 槲皮素/g)和 IC 50 (15.25 μg/ml)在A. racemosus的甲醇提取物中高于n-己烷、氯仿和乙酸乙酯萃取物。HPTLC 与化学衍生物(DPPH•、茴香醛/硫酸和氯化铁)联用,用于评估抗氧化活性和植物甾醇、萜类化合物和多酚含量的存在。提取物中 100* 保留因子 = 58、68、74 和 65 的相同化合物具有抗氧化活性。联用 HPTLC 结合效应导向分析和衰减全反射 - 傅里叶变换红外光谱,可以快速表征活性化合物。来自衰减全反射率的谱带分析确定杨梅素、槲皮素、香豆酸和咖啡酸负责抗氧化活性。
更新日期:2021-08-18
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