Journal of Molecular Neuroscience ( IF 3.1 ) Pub Date : 2021-08-19 , DOI: 10.1007/s12031-021-01884-w Anna Sergeevna Piatoikina 1 , Anastasia Alexandrovna Lyakhova 2 , Ilya Vladimirovich Semennov 2 , Tatyana Vladimirovna Zhilyaeva 2 , Olga Vladimirovna Kostina 2 , Ekaterina Sergeevna Zhukova 3 , Tatyana Grigorievna Shcherbatyuk 3, 4 , Evgeny Dmitrievich Kasyanov 5 , Anna Sergeevna Blagonravova 2 , Galina Elevna Mazo 5
Schizophrenia is considered a multifactorial disease, where one of the pathogenetic links is oxidative stress; however, the results of studies are often contradictory, largely due to significant heterogeneity among study methods. The present study was undertaken to compare the levels of oxidative stress markers in the peripheral blood of patients with a first episode of schizophrenia (FES) and in healthy volunteers (HV). The study included 50 patients with FES and 37 HV. Blood samples were collected for spectrophotometric assessment of reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), aldehyde-2,4-dinitrophenylhydrazone (ADNPH), and ketone-2,4-dinitrophenylhydrazone (KDNPH) in blood plasma. Results showed that in patients with FES compared with HV, a significant decrease in CAT activity and an increase in oxidative modification of proteins (OMP) were found. In both groups, a significant increase in the level of MDA with age was revealed. In patients, the GSH plasma level was inversely proportional to the ADNPH level, and SOD activity was directly proportional to the KDNPH level. In volunteers there was no such correlation; however, there was a direct correlation between CAT activity and the levels of OMP and MDA. In both groups, a moderate direct correlation between peroxidation products was observed. The results confirm that a redox imbalance (a deficiency of antioxidants, in particular CAT, and excess OMP) may be a pathogenetic link in schizophrenia, which is manifested already at an early stage of the disease.
中文翻译:
首发精神分裂症患者抗氧化缺乏与蛋白质和脂质过氧化产物水平的关系
精神分裂症被认为是一种多因素疾病,其中一个致病环节是氧化应激;然而,研究结果往往相互矛盾,这主要是由于研究方法之间存在显着的异质性。本研究旨在比较首发精神分裂症患者 (FES) 和健康志愿者 (HV) 外周血中氧化应激标志物的水平。该研究包括 50 名 FES 和 37 HV 患者。采集血样用于分光光度法评估还原型谷胱甘肽 (GSH)、超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、丙二醛 (MDA)、醛-2,4-二硝基苯腙 (ADNPH) 和酮-2,4-二硝基苯腙 ( KDNPH)在血浆中。结果表明,在 FES 患者中,与 HV 相比,发现 CAT 活性显着降低和蛋白质氧化修饰 (OMP) 增加。在两组中,发现MDA水平随着年龄的增长而显着增加。在患者中,GSH 血浆水平与 ADNPH 水平成反比,SOD 活性与 KDNPH 水平成正比。在志愿者中没有这种相关性;然而,CAT 活性与 OMP 和 MDA 水平之间存在直接相关性。在两组中,观察到过氧化产物之间存在中等程度的直接相关性。结果证实,氧化还原失衡(抗氧化剂缺乏,特别是 CAT 和过量的 OMP)可能是精神分裂症的致病联系,这已经在疾病的早期阶段表现出来。随着年龄的增长,MDA水平显着增加。在患者中,GSH 血浆水平与 ADNPH 水平成反比,SOD 活性与 KDNPH 水平成正比。在志愿者中没有这种相关性;然而,CAT 活性与 OMP 和 MDA 水平之间存在直接相关性。在两组中,观察到过氧化产物之间存在中等程度的直接相关性。结果证实,氧化还原失衡(抗氧化剂缺乏,特别是 CAT 和过量的 OMP)可能是精神分裂症的致病联系,这已经在疾病的早期阶段表现出来。随着年龄的增长,MDA水平显着增加。在患者中,GSH 血浆水平与 ADNPH 水平成反比,SOD 活性与 KDNPH 水平成正比。在志愿者中没有这种相关性;然而,CAT 活性与 OMP 和 MDA 水平之间存在直接相关性。在两组中,观察到过氧化产物之间存在中等程度的直接相关性。结果证实,氧化还原失衡(抗氧化剂缺乏,特别是 CAT 和过量的 OMP)可能是精神分裂症的致病联系,这已经在疾病的早期阶段表现出来。在志愿者中没有这种相关性;然而,CAT 活性与 OMP 和 MDA 水平之间存在直接相关性。在两组中,观察到过氧化产物之间存在中等程度的直接相关性。结果证实,氧化还原失衡(抗氧化剂缺乏,特别是 CAT 和过量的 OMP)可能是精神分裂症的致病联系,这已经在疾病的早期阶段表现出来。在志愿者中没有这种相关性;然而,CAT 活性与 OMP 和 MDA 水平之间存在直接相关性。在两组中,观察到过氧化产物之间存在中等程度的直接相关性。结果证实,氧化还原失衡(抗氧化剂缺乏,特别是 CAT 和过量的 OMP)可能是精神分裂症的致病联系,这已经在疾病的早期阶段表现出来。