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Protein kinase C theta (Prkcq) affects nerve degeneration and regeneration through the c-fos and c-jun pathways in injured rat sciatic nerves
Experimental Neurology ( IF 5.3 ) Pub Date : 2021-08-19 , DOI: 10.1016/j.expneurol.2021.113843
Yi Wang 1 , Nannan Gao 1 , Yumei Feng 1 , Min Cai 2 , Yuting Li 1 , Xi Xu 3 , Huanhuan Zhang 1 , Dengbing Yao 1
Affiliation  

Background

Previous finding using DNA microarray and bioinformatics analysis, we have reported some key factors which regulated gene expression and signaling pathways in injured sciatic nerve during Wallerian Degeneration (WD). This research is focused on protein kinase C theta (Prkcq) participates in the regulation of the WD process.

Methods

In this study, we explored the molecular mechanism by which Prkcq in Schwann cells (SCs) affects nerve degeneration and regeneration in vivo and in vitro after rat sciatic nerve injury.

Results

Study of the cross-sectional model showed that Prkcq expression decreased significantly during sciatic nerve repair. Functional analysis showed that upregulation and downregulation of Prkcq could affect the proliferation, migration and apoptosis of Schwann cells and lead to the expression of related factors through the activation of the β-catenin, c-fos, and p-c-jun/c-jun pathways.

Conclusion

The study provides insights into the role of Prkcq in early WD during peripheral nerve degeneration and/or regeneration.



中文翻译:

蛋白激酶 C theta (Prkcq) 通过 c-fos 和 c-jun 通路影响受伤大鼠坐骨神经的神经变性和再生

背景

先前使用 DNA 微阵列和生物信息学分析的发现,我们已经报道了在沃勒变性 (WD) 期间调节受损坐骨神经中基因表达和信号通路的一些关键因素。这项研究的重点是蛋白激酶 C theta (Prkcq) 参与调控 WD 过程。

方法

在这项研究中,我们探索了雪旺细胞 (SCs) 中的 Prkcq 在大鼠坐骨神经损伤后影响体内和体外神经变性和再生的分子机制。

结果

横断面模型研究表明,在坐骨神经修复过程中,Prkcq 表达显着降低。功能分析表明,Prkcq的上调和下调可影响雪旺细胞的增殖、迁移和凋亡,并通过激活β-catenin、c-fos和pc-jun/c-jun通路导致相关因子的表达.

结论

该研究提供了对 Prkcq 在周围神经变性和/或再生过程中早期 WD 的作用的见解。

更新日期:2021-08-25
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