Tyrosol and hydroxytyrosol derived from virgin olive oil and olives extract, have wide applications both as functional food components and as nutraceuticals. However, they have low bioavailability due to their low absorption and high metabolism in human liver and small intestine. Acetylation of tyrosol and hydroxytyrosol can effectively improve their bioavailability and thus increase their potential use in the food and cosmeceutical industries. There is no report on the bioproductin of tyrosol acetate and hydroxytyrosol acetate so far. Thus, it is of great significance to develop microbial cell factories for achieving tyrosol acetate or hydroxytyrosol acetate biosynthesis. In this study, a de novo biosynthetic pathway for the production of tyrosol acetate and hydroxytyrosol acetate was constructed in Escherichia coli. First, an engineered E. coli that allows production of tyrosol from simple carbon sources was established. Four aldehyde reductases were compared, and it was found that yeaE is the best aldehyde reductase for tyrosol accumulation. Subsequently, the pathway was extended for tyrosol acetate production by further overexpression of alcohol acetyltransferase ATF1 for the conversion of tyrosol to tyrosol acetate. Finally, the pathway was further extended for hydroxytyrosol acetate production by overexpression of 4-hydroxyphenylacetate 3-hydroxylase HpaBC.

源自初榨橄榄油和橄榄提取物的酪醇和羟基酪醇作为功能性食品成分和营养品具有广泛的应用。然而，由于它们在人肝脏和小肠中的低吸收和高代谢，它们的生物利用度低。酪醇和羟基酪醇的乙酰化可以有效提高它们的生物利用度，从而增加它们在食品和药妆行业的潜在用途。目前还没有关于醋酸酪醇和羟基酪醇醋酸酯的生物制品的报道。因此，开发微生物细胞工厂对于实现醋酸酪醇或羟基酪醇醋酸盐的生物合成具有重要意义。在本研究中，构建了一条生产醋酸酪醇和羟基酪醇醋酸盐的从头生物合成途径。大肠杆菌。首先，建立了一种允许从简单碳源生产酪醇的工程大肠杆菌。对四种醛还原酶进行了比较，发现yeaE是酪醇积累的最佳醛还原酶。随后，通过进一步过表达乙醇乙酰转移酶 ATF1 将酪醇转化为醋酸酪醇，该途径被扩展到醋酸酪醇的生产。最后，通过 4-羟基苯乙酸 3-羟化酶 HpaBC 的过度表达，进一步扩展了生产羟基酪醇乙酸酯的途径。