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Determination of chlorogenic acid in Eucommia ulmoides Oliver extracts by near-infrared spectroscopy
Spectroscopy Letters ( IF 1.7 ) Pub Date : 2021-08-17 , DOI: 10.1080/00387010.2021.1963286
Mayowa Oyebanji 1, 2 , Xuan Dong 1, 2 , Qianqian Zhang 1, 2 , Guohua Yao 1 , Qing Huang 1, 2
Affiliation  

Abstract

Eucommia ulmoides Oliver shows various medicinal functions in both in-vitro and in-vivo studies, and its chlorogenic acid component has been indicated as one of the major bioactive compounds exerting pharmacological effects. At present, the high-performance liquid chromatography technique is the conventional method used for the quantification of chlorogenic acids, but it requires expensive instrumentation and complicated operation. In the current research, near-infrared spectroscopy was employed for the quantitative analysis of chlorogenic acid in Eucommia ulmoides. The quantification model was established based on near-infrared spectroscopy spectra for analysis of chlorogenic acids in the ethanol-aqueous extract from Eucommia ulmoides leaf. A partial least squares regression was employed for the quantitative model development and test validation was adopted to validate the regression model. The optimized ranges for the quantitative model are 4381.7–4034.6 cm−1 and 6815.5–6471.6 cm−1 with the pretreatment of data using the first derivative and vector normalization of the near-infrared spectra, with the rank = 5, coefficient of determination = 0.8843, residual predictive deviation = 3.18, and root mean square error of prediction = 1.37. This work, therefore, provides an effective and convenient method for the rapid quantitative analysis of chlorogenic acid content in Eucommia leaf.



中文翻译:

近红外光谱法测定杜仲提取物中绿原酸的含量

摘要

杜仲在体外体内研究中显示出多种药用功能,其绿原酸成分已被证明是发挥药理作用的主要生物活性化合物之一。目前,高效液相色谱技术是绿原酸定量的常规方法,但仪器昂贵,操作复杂。目前的研究采用近红外光谱技术对杜仲中的绿原酸进行定量分析。建立了基于近红外光谱的定量模型,用于分析水杨酸乙醇提取物中的绿原酸。杜仲叶。采用偏最小二乘回归进行定量模型开发,并采用测试验证来验证回归模型。定量模型的优化范围为 4381.7-4034.6 cm -1和 6815.5-6471.6 cm -1使用近红外光谱的一阶导数和矢量归一化对数据进行预处理,秩 = 5,决定系数 = 0.8843,残差预测偏差 = 3.18,预测均方根误差 = 1.37。因此,该工作为杜仲叶中绿原酸含量的快速定量分析提供了一种有效、便捷的方法。

更新日期:2021-10-09
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