Aquatic Toxicology ( IF 4.5 ) Pub Date : 2021-08-19 , DOI: 10.1016/j.aquatox.2021.105940 Wenbin Liu 1 , Xiaoya Sun 1 , Wenwen Sun 1 , Anmo Zhou 1 , Ruoqun Li 1 , Bin Wang 2 , Xun Li 2 , Chuncai Yan 1
Non-biting midges are dominant species in aquatic systems and often used for studying the toxicological researches of insecticides. ATP-binding cassette (ABC) transporters represent the largest known members in detoxification genes but is little known about their function in non-biting midges. Here, we selected Propsilocerus akamusi, widespread in urban streams, to first uncover the gene structure, location, characteristics, and phylogenetics of chironomid ABC transporters at genome-scale. Fifty-seven ABC transporter genes are located on four chromosomes, including eight subfamilies (ABCA-H). The ABCC, ABCG, and ABCH subfamilies experienced the duplication events to different degrees. The study showed that expression of the PaABCG17 gene is uniquely significantly elevated, with deltamethrin concentration increasing (1, 4, and 20 ug/L) both in RNA-seq and qPCR results. Additionally, the ABC transporter members of other six chironomids with assembled genomes are first described and used to investigate the characteristic of those living in the different adverse habitats. The ABC transporter frame for Propsilocerus akamusi and its transcriptomic results lay an important foundation for providing valuable resources for understanding the ABC transporter function in insecticide toxification of this species as well as those of other non-biting midges. The PaABCG17 gene is shown to play an important role in deltamethrin detoxification, and it functions need to be further investigated and might be used in the management of insecticide-resistance in chironomid adults.
中文翻译:
ATP 结合盒 (ABC) 转运蛋白基因家族的全基因组分析及其与非叮咬蚊蠓中溴氰菊酯耐受性相关的表达谱
不咬人的蠓是水生系统中的优势种,常用于研究杀虫剂的毒理学研究。ATP 结合盒 (ABC) 转运蛋白代表解毒基因中最大的已知成员,但对其在非咬人蠓中的功能知之甚少。在这里,我们选择了普遍存在于城市河流中的Propsilocerus akamusi,首先在基因组规模上揭示摇蚊 ABC 转运蛋白的基因结构、位置、特征和系统发育。57 个 ABC 转运蛋白基因位于四个染色体上,包括八个亚家族 (ABCA-H)。ABCC、ABCG 和 ABCH 亚家族经历了不同程度的重复事件。研究表明,PaABCG17 的表达基因显着升高,在 RNA-seq 和 qPCR 结果中溴氰菊酯浓度增加(1、4 和 20 ug/L)。此外,首先描述了具有组装基因组的其他六种摇蚊的 ABC 转运蛋白成员,并用于研究生活在不同不利栖息地的人的特征。Propsilocerus akamusi的 ABC 转运蛋白框架及其转录组学结果为了解 ABC 转运蛋白在该物种以及其他非叮咬蠓的杀虫剂毒化中的功能提供了宝贵的资源。该PaABCG17 基因在溴氰菊酯解毒中发挥重要作用,其功能需要进一步研究,并可能用于控制摇蚊成虫的杀虫剂抗性。