In this study, the effect of protonectin as an antimicrobial peptide in its encapsulated form into PEG-PCL Nano micelle on bioability of human cancer lung of A549 was investigated. By applying 12 µg/ml and 50 µg/ml of the peptide in its encapsulated form and post 24 h and 48 h cell treatment, highest cytotoxicity on A549 was noticed. Biodegradable and biocompatible micelles were made by connecting poly (ethylene glycol) and poly (Ɛ-caprolactone) (PEG-PCL). NMR and FT-IR techniques were applied to characterize PEG-PCL micelle, while the properties of encapsulated peptide into PEG-PCL were further researched by using HPLC, scanning electron microscope (SEM), dynamic light scattering (DLS) as well as zeta potential. The impacts of PEG-PCL encapsulated protonectin with yield loading of 89.7% on the gene expression pattern of metastatic factors BMI-1 and CD44 were examined by the real-time RT PCR. Outcomes exhibited that BMI1 gene expression was down-regulated at a higher level compared to that of CD44 at the concentration of 12 µg/ml, and post 24 and 48 h. In conclusion, our finding supports a novel drug delivery system that can be applicable to enhance the solubility of insoluble drugs, has harmless effects on cancer cells on its own, and is cytotoxic in its encapsulated form.

在这项研究中，研究了原连接蛋白作为一种抗菌肽，将其封装在 PEG-PCL 纳米胶束中对 A549 人肺癌肺生物活性的影响。通过应用 12 µg/ml 和 50 µg/ml 的封装形式的肽并在细胞处理 24 小时和 48 小时后，注意到对 A549 的细胞毒性最高。通过连接聚（乙二醇）和聚（Ɛ-己内酯）（PEG-PCL）制成可生物降解和生物相容性胶束。NMR和FT-IR技术用于表征PEG-PCL胶束，同时通过使用HPLC、扫描电子显微镜（SEM）、动态光散射（DLS）以及zeta电位进一步研究了包封到PEG-PCL中的肽的性质. PEG-PCL 封装的原连接蛋白的影响，屈服载荷为 89。7% 的转移因子 BMI-1 和 CD44 的基因表达模式通过实时 RT PCR 检查。结果表明，与 CD44 相比，在 12 µg/ml 浓度下，以及 24 和 48 小时后，BMI1 基因表达下调水平更高。总之，我们的发现支持了一种新的药物递送系统，该系统可用于提高不溶性药物的溶解度，其本身对癌细胞无害，并且其封装形式具有细胞毒性。