This study investigated the antifungal activity and possible mechanisms of an extract from Lysurus mokusin (L134) against Pestalotiopsis neglecta. The effect of Lysurus mokusin (L134) on the enzyme activity, membrane permeability, and cell wall integrity of P. neglecta was assayed. Besides, we conducted a gas chromatography/mass spectrometry (GC–MS) to identify the individual compounds in Lysurus mokusin (L134). Pinus sylvestris var. mongolica Litv was used as the study object to evaluate the prevention and treatment effect of Lysurus mokusin L134 extract against P. neglecta (black spot needle blight). The results showed that Lysurus mokusin (L134) has a potent inhibitory effect against P. neglecta mycelial growth, and the inhibition rate is positively correlated with the concentration of Lysurus mokusin (L134). Besides, Lysurus mokusin (L134) can effectively inhibit spore germination in P. neglecta. Scanning electron microscopy (SEM) showed that after Lysurus mokusin (L134) treatment, the mycelia assumed an inward concave shape, became distorted, atrophied, and swollen. Evaluation of the antifungal mechanism indicated that Lysurus mokusin (L134) reduces cell wall integrity and increases cell membrane permeability. Furthermore, the effect of biocontrol revealed that L134 could effectively control black spot needle blight of Pinus sylvestris var. mongolica. GC–MS analysis of the Lysurus mokusin (L134) fraction obtained from n-butanol revealed d-glucitol,hexaacetate, l-( +)-rhamnose,aldononitrile,tetraacetate are the major compounds. The present study is the first report that these compounds have fungistatic activity. Overall, this study suggests that Lysurus mokusin (L134) extract can potentially be used as a fungicide for the sustainable management of plant diseases.

本研究调查了来自Lysurus mokusin (L134)的提取物对Pestalotiopsis ignorea的抗真菌活性和可能的​​机制。的效果Lysurus mokusin（L134）上的酶活性，膜的渗透性，和细胞壁完整性P. neglecta进行测定。此外，我们进行了气相色谱/质谱 (GC-MS) 来鉴定Lysurus mokusin (L134) 中的单个化合物。樟子松变种。樟子松LITV用作研究对象以评估的预防和治疗效果Lysurus mokusin L134提取针对P. neglecta（黑斑病针枯病）. 结果表明，Lysurus mokusin (L134) 对P. ignorea菌丝体生长有很强的抑制作用，抑制率与Lysurus mokusin (L134)的浓度呈正相关。此外，Lysurus mokusin (L134) 能有效抑制P. ignorea 孢子萌发。扫描电子显微镜（SEM）显示，Lysurus mokusin (L134) 处理后，菌丝呈向内凹形，变形、萎缩和肿胀。抗真菌机制的评估表明，Lysurus mokusin(L134) 降低细胞壁完整性并增加细胞膜通透性。此外，生防效果表明L134能有效防治樟子松黑斑针叶枯病。蒙古。从正丁醇中获得的Lysurus mokusin (L134) 馏分的GC-MS 分析显示 d-葡萄糖醇、六乙酸酯、l-(+)-鼠李糖、醛酮腈、四乙酸酯是主要化合物。本研究是首次报道这些化合物具有抑菌活性。总的来说，这项研究表明，Lysurus mokusin (L134) 提取物有可能用作植物病害可持续管理的杀菌剂。