Abstract

The heterotrimeric (αβγ) translation initiation factor 2 of archaea and eukaryotes (a/eIF2) supplies the P-site of the ribosome with the initiation tRNA. Its two subunits (β and γ) contain the Cys2‑Cys2 motif, which is capable of forming a stable zinc finger structure in the presence of zinc ions. In this work, comparative analysis of the fragments containing Cys2‑Cys2 motifs in the aIF2β and aIF2γ structures from different organisms was carried out and their environments in crystals was analyzed. Based on the obtained data, a conclusion was made that the conformation and role of these fragments in the β- and γ-subunits of the aIF2 are different.

中文翻译：

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含有 Cys2‑Cys2 基序的古菌翻译起始因子 2 片段的结构和功能

摘要

古细菌和真核生物 (a/eIF2) 的异源三聚体 (αβγ) 翻译起始因子 2 为核糖体的 P 位点提供起始 tRNA。它的两个亚基（β 和 γ）包含 Cys2‑Cys2 基序，能够在锌离子存在下形成稳定的锌指结构。在这项工作中，对来自不同生物体的 aIF2β 和 aIF2γ 结构中含有 Cys2-Cys2 基序的片段进行了比较分析，并分析了它们在晶体中的环境。根据获得的数据得出结论，这些片段在aIF2的β-和γ-亚基中的构象和作用是不同的。