N6-methyladenosine (m6A) plays an important role in the gene expression regulation. Previously, we found an ortholog of Arabidopsis LBD15 that showed xylem preferential expression and involved in leaf development in Poplar 84 K. In order to investigate whether m6A modification affects the function of LBD15, the m6A-immunoprecipitation sequence and the matched input RNA sequence for non-transgenic plants (CK) and the LBD15 overexpression (LBD15-oe) plants were compared and analyzed. As a result, 7,156 differential m6A peaks were identified, with 2,896 upregulated m6A peaks and 4,260 downregulated m6A peaks. Correlation analysis of differential expression genes and differential m6A peaks indicated that a total of 119 differently methylated genes showed a negative correlation with the differentially expressed genes. Among them, Nudix hydrolase, LRR receptor-like serine/threonine-protein kinase, tubulin, vacuole membrane protein KMS1, and MYB family transcription factor PHL11 may be involved in the posttranscriptional gene regulation in LBD15 overexpression plants. The expression of ten m6A-modified genes was validated by qRT-PCR. Our results will provide a basis for the further elucidation of the regulatory mechanism of m6A modification and the epigenetic regulation of LBD15.

N6-甲基腺苷（m6A）在基因表达调控中起重要作用。此前，我们发现了拟南芥 LBD15 的直向同源物，其在杨树 84 K 中表现出木质部优先表达并参与叶发育。 -转基因植物(CK)和LBD15过表达(LBD15-oe)植物进行了比较和分析。结果，鉴定了 7,156 个差异 m6A 峰，其中 2,896 个上调的 m6A 峰和 4,260 个下调的 m6A 峰。差异表达基因与差异m6A峰的相关分析表明，共有119个不同甲基化基因与差异表达基因呈负相关。其中，Nudix水解酶、LRR 受体样丝氨酸/苏氨酸蛋白激酶、微管蛋白、液泡膜蛋白 KMS1 和 MYB 家族转录因子 PHL11 可能参与 LBD15 过表达植物的转录后基因调控。通过 qRT-PCR 验证了十个 m6A 修饰基因的表达。我们的研究结果将为进一步阐明 m6A 修饰的调控机制和 LBD15 的表观遗传调控提供基础。