Colorectal cancer is the main cause of human death due to cancer. This fact could be due to the insufficiency of early diagnosis or poor therapeutic strategies. Various molecular tools have been utilized in studies to assess their potentials as diagnostic biomarkers or determining factors in precision medicine. Among these molecules, long non-coding RNAs (lncRNA) have been emerging as accurate and potent transcripts to improve the detection of cancer. The overexpressed lncRNAs could also be deeply studied as the molecules for the targeted therapy in different malignancies, in particular colorectal cancer. Thus, we utilized an unbiased approach to select the up-regulated lncRNAs in colon adenocarcinoma via analyzing the TCGA dataset. Then, we validated the overexpression of two first-ranked lncRNAs, i.e., NPSR1-AS1 and TLX1NB, in our in-house colorectal cancer samples as compared to the paired adjacent normal tissues. The analyses revealed that these lncRNAs could significantly distinguish the tumor against the normal samples. The results may have implications in the early diagnosis and targeted therapy of colorectal cancer.

中文翻译：

---

高表达的 TLX1NB 和 NPSR1-AS1 lncRNA 可作为结直肠癌的诊断工具。

结直肠癌是导致人类因癌症死亡的主要原因。这一事实可能是由于早期诊断不足或治疗策略不佳所致。各种分子工具已被用于研究中，以评估它们作为诊断生物标志物或精准医学决定因素的潜力。在这些分子中，长链非编码 RNA (lncRNA) 已作为准确而有效的转录本出现，以改善癌症的检测。过表达的 lncRNA 也可以作为靶向治疗不同恶性肿瘤，特别是结直肠癌的分子进行深入研究。因此，我们利用一种公正的方法通过分析 TCGA 数据集来选择结肠腺癌中上调的 lncRNA。然后，我们验证了两个排名第一的 lncRNA，即 NPSR1-AS1 和 TLX1NB 的过表达，在我们的内部结直肠癌样本中，与配对的相邻正常组织相比。分析表明，这些 lncRNA 可以显着区分肿瘤与正常样本。该结果可能对结直肠癌的早期诊断和靶向治疗产生影响。