The applications of exogenous hormones in different species for the induction of oocyte production, final oocyte maturation (FOM), and spawning for their reproduction is getting more attention day by day. The current preliminary research work was carried out to induce breeding in Clarias gariepinus, commonly known as African catfish, imported from Thailand. Single doses of two hormones as Ovaprim and human menopausal gonadotropin (hMG) were used and the research work was carried out at Muzaffargarh Fish Hatchery Punjab, Pakistan. A total of twenty-four (n = 24) C. gariepinus were selected having body weight approximately 2 kg and divided into two main groups based on gender as male (n = 12) and female (n = 12). For milt collection, all males were treated with Ovaprim 0.5 mg/kg body weight (b.w.) and female fish were divided into three groups as A, B, and C with four (n = 4) fish in each group. Group A was injected with only normal saline (control group) while fish in group B and group C were treated with hMG at 0.5 mg/kg b.w. and Ovaprim 0.5 mg/kg b.w., respectively. Then, after 6 h of hormone injections until 48 h, spawned eggs, eggs’ weight, fertilization rate, hatching rate, survival rate, fecundity, and deformed larvae were investigated. The results revealed that Ovaprim injection significantly (p < 0.05) modulate the reproductive parameters in group C while no breeding was induced in both control and hMG-treated groups. Hence, it could be concluded that Ovaprim has the potential to induce breeding in African catfish, while in the current study, hMG failed to induce breeding. However, trials at large scales are required to further explore the effect of different doses of both tested hormones by increasing the treated subjects particularly in Pakistani fish farms.

外源激素在不同物种中用于诱导卵母细胞产生、最终卵母细胞成熟（FOM）和产卵繁殖的应用日益受到关注。目前的初步研究工作是在从泰国进口的通常被称为非洲鲶鱼的Clarias gariepinus 中进行诱导繁殖。使用单剂量的两种激素，如 Ovaprim 和人类更年期促性腺激素 (hMG)，研究工作在巴基斯坦旁遮普的 Muzaffargarh Fish Hatchery 进行。总共选择了 24 ( n = 24) C. gariepinus体重约 2 kg 并根据性别分为雄性 ( n  = 12) 和雌性 ( n = 12）。对于鱼精收集，所有雄鱼都用 Ovaprim 0.5 mg/kg 体重 (bw) 处理，雌鱼分为 A、B 和 C 三组，每组四条 ( n  = 4) 鱼。A 组仅注射生理盐水（对照组），而 B 组和 C 组的鱼分别注射 0.5 毫克/千克体重的 hMG 和 0.5 毫克/千克体重的 Ovaprim。然后，在注射激素 6 小时后至 48 小时，调查产卵、卵重、受精率、孵化率、成活率、繁殖力和畸形幼虫。结果显示，Ovaprim注射液显着（p < 0.05) 调节 C 组的繁殖参数，而对照组和 hMG 治疗组均未诱导繁殖。因此，可以得出结论，Ovaprim 具有诱导非洲鲶鱼繁殖的潜力，而在目前的研究中，hMG 未能诱导繁殖。然而，需要进行大规模试验，通过增加治疗对象，特别是在巴基斯坦养鱼场，进一步探索不同剂量的两种测试激素的影响。