Porous Titanium-6Aluminum-4Vanadium scaffolds made by electron beam-based additive manufacturing (AM) have emerged as state-of-the-art implant devices. However, there is still limited knowledge on how they influence the osteogenic differentiation of bone marrow-derived mesenchymal stromal cells (BMSCs). In this study, BMSCs are cultured on such porous scaffolds to determine how the scaffolds influence the osteogenic differentiation of the cells. The scaffolds are biocompatible, as revealed by the increasing cell viability. Cells are evenly distributed on the scaffolds after 3 days of culturing followed by an increase in bone matrix development after 21 days of culturing. qPCR analysis provides insight into the cells’ osteogenic differentiation, where RUNX2 expression indicate the onset of differentiation towards osteoblasts. The COL1A1 expression suggests that the differentiated osteoblasts can produce the osteoid. Alkaline phosphatase staining indicates an onset of mineralization at day 7 in OM. The even deposits of calcium at day 21 further supports a successful bone mineralization. This work shines light on the interplay between AM Ti64 scaffolds and bone growth, which may ultimately lead to a new way of creating long lasting bone implants with fast recovery times.

由基于电子束的增材制造 (AM) 制成的多孔 Titanium-6Aluminum-4Vanadium 支架已成为最先进的植入设备。然而，关于它们如何影响骨髓间充质基质细胞（BMSCs）的成骨分化的知识仍然有限。在这项研究中，在这种多孔支架上培养 BMSC，以确定支架如何影响细胞的成骨分化。支架具有生物相容性，正如细胞活力增加所揭示的那样。培养 3 天后细胞均匀分布在支架上，培养 21 天后骨基质发育增加。qPCR 分析可以深入了解细胞的成骨分化，其中RUNX2表达表明开始向成骨细胞分化。COL1A1表达表明分化的成骨细胞可以产生类骨质。碱性磷酸酶染色表明 OM 在第 7 天开始矿化。第 21 天钙的均匀沉积进一步支持成功的骨矿化。这项工作揭示了 AM Ti64 支架与骨骼生长之间的相互作用，这可能最终导致一种新的方法来制造具有快速恢复时间的长效骨植入物。