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Endophytic bacterial community diversity in two citrus cultivars with different citrus canker disease resistance.
Archives of Microbiology ( IF 2.8 ) Pub Date : 2021-08-18 , DOI: 10.1007/s00203-021-02530-0
Bing Liu 1 , Jiahao Lai 1 , Simeng Wu 1 , Junxi Jiang 1 , Weigang Kuang 1
Affiliation  

The selective infection of Xanthomonas citri pv. citri (Xcc) to citrus cultivars is universally known, but the relationship between endophytic bacteria and the resistance of host variety to canker disease remains unclear. In this study, endophytic bacterial populations of two citrus cultivars-the resistant satsuma mandarin and the susceptible Newhall navel orange-were analyzed through high-throughput sequencing. The results showed that endophytic bacterial community of satsuma mandarin was more abundant than that of Newhall navel orange. In addition, bacterial abundance was the highest in the spring samples, followed by that in summer and winter samples, in both the varieties. In all samples, the predominant phyla were Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes; the major genera were Bacillus and Stenotrophomonas, and the main species was Bacillus subtilis. According to the analysis of the predominant bacteria in the two citrus cultivars, B. subtilis with potential antagonistic characteristics against Xcc existed universally in all samples. However, the susceptible Newhall navel oranges were abundant in Bacillus subtilis and had a relatively large number of canker-causing cooperative bacteria such as Stenotrophomonas. The results suggested that endophytic bacterial community of the two citrus cultivars had some differences based on the season or plant tissue, and these differences were mainly in the quantity of bacteria, affecting citrus canker disease occurrence. In conclusion, the differences in endophytic bacteria on citrus cultivars might be related to host resistance or susceptibility to citrus canker disease.

中文翻译:

两个具有不同柑橘溃疡病抗性的柑橘品种的内生细菌群落多样性。

柑橘黄单胞菌 pv 的选择性感染。citri (Xcc) 对柑橘品种的影响是众所周知的,但内生细菌与宿主品种对溃疡病的抗性之间的关系仍不清楚。在这项研究中,通过高通量测序分析了两个柑橘品种——抗性蜜柑和易感纽霍尔脐橙——的内生细菌种群。结果表明,温州蜜柑的内生菌群落比纽荷尔脐橙丰富。此外,两个品种的细菌丰度在春季样品中最高,其次是夏季和冬季样品。在所有样本中,主要的门是变形菌门、厚壁菌门、放线菌门和拟杆菌门;主要属是芽孢杆菌属和窄养单胞菌属,主要菌种为枯草芽孢杆菌。根据对两个柑橘品种优势菌的分析,对Xcc具有潜在拮抗特性的枯草芽孢杆菌普遍存在于所有样品中。然而,易感的纽霍尔脐橙中枯草芽孢杆菌含量丰富,并且有较多的致溃疡合作菌,如嗜食单胞菌。结果表明,两个柑橘品种的内生细菌群落因季节或植物组织的不同而存在一定差异,这些差异主要体现在细菌数量上,影响柑橘溃疡病的发生。总之,柑橘品种内生细菌的差异可能与宿主对柑橘溃疡病的抗性或易感性有关。根据对两个柑橘品种优势菌的分析,对Xcc具有潜在拮抗特性的枯草芽孢杆菌普遍存在于所有样品中。然而,易感的纽霍尔脐橙中枯草芽孢杆菌含量丰富,并且有较多的致溃疡合作菌,如嗜食单胞菌。结果表明,两个柑橘品种的内生细菌群落因季节或植物组织的不同而存在一定差异,这些差异主要体现在细菌数量上,影响柑橘溃疡病的发生。总之,柑橘品种内生细菌的差异可能与宿主对柑橘溃疡病的抗性或易感性有关。根据对两个柑橘品种优势菌的分析,对Xcc具有潜在拮抗特性的枯草芽孢杆菌普遍存在于所有样品中。然而,易感的纽霍尔脐橙中枯草芽孢杆菌含量丰富,并且有较多的致溃疡合作菌,如嗜食单胞菌。结果表明,两个柑橘品种的内生细菌群落因季节或植物组织的不同而存在一定差异,这些差异主要体现在细菌数量上,影响柑橘溃疡病的发生。总之,柑橘品种内生细菌的差异可能与宿主对柑橘溃疡病的抗性或易感性有关。具有针对 Xcc 的潜在拮抗特性的枯草杆菌普遍存在于所有样品中。然而,易感的纽霍尔脐橙中枯草芽孢杆菌含量丰富,并且有较多的致溃疡合作菌,如嗜食单胞菌。结果表明,两个柑橘品种的内生细菌群落因季节或植物组织的不同而存在一定差异,这些差异主要体现在细菌数量上,影响柑橘溃疡病的发生。总之,柑橘品种内生细菌的差异可能与宿主对柑橘溃疡病的抗性或易感性有关。具有针对 Xcc 的潜在拮抗特性的枯草杆菌普遍存在于所有样品中。然而,易感的纽霍尔脐橙中枯草芽孢杆菌含量丰富,并且有较多的致溃疡合作菌,如嗜食单胞菌。结果表明,两个柑橘品种的内生细菌群落因季节或植物组织的不同而存在一定差异,这些差异主要体现在细菌数量上,影响柑橘溃疡病的发生。总之,柑橘品种内生细菌的差异可能与宿主对柑橘溃疡病的抗性或易感性有关。易感的纽荷尔脐橙富含枯草芽孢杆菌,并含有相对较多的致溃疡合作菌,如寡养单胞菌。结果表明,两个柑橘品种的内生细菌群落因季节或植物组织的不同而存在一定差异,这些差异主要体现在细菌数量上,影响柑橘溃疡病的发生。总之,柑橘品种内生细菌的差异可能与宿主对柑橘溃疡病的抗性或易感性有关。易感的纽荷尔脐橙富含枯草芽孢杆菌,并含有相对较多的致溃疡合作菌,如寡养单胞菌。结果表明,两个柑橘品种的内生细菌群落因季节或植物组织的不同而存在一定差异,这些差异主要体现在细菌数量上,影响柑橘溃疡病的发生。总之,柑橘品种内生细菌的差异可能与宿主对柑橘溃疡病的抗性或易感性有关。而这些差异主要体现在细菌的数量上,影响柑橘溃疡病的发生。总之,柑橘品种内生细菌的差异可能与宿主对柑橘溃疡病的抗性或易感性有关。而这些差异主要体现在细菌的数量上,影响柑橘溃疡病的发生。总之,柑橘品种内生细菌的差异可能与宿主对柑橘溃疡病的抗性或易感性有关。
更新日期:2021-08-18
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