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SABRE-enhanced real-time pure shift NMR spectroscopy
Magnetic Resonance in Chemistry ( IF 2 ) Pub Date : 2021-08-17 , DOI: 10.1002/mrc.5206
Daniel A Taylor 1 , Louise S Natrajan 1 , Mathias Nilsson 1 , Ralph W Adams 1
Affiliation  

Pure shift nuclear magnetic resonance (NMR) methods suppress the effect of homonuclear scalar couplings to produce NMR spectra consisting solely of a single signal for each chemically distinct site. They are increasingly relied upon for analysis of complex molecules and mixtures as they overcome the extensive signal overlap that complicates proton NMR spectra of all but the simplest species. Current broadband pure shift methodologies for 1D proton spectra suffer from reduced sensitivity compared with their conventional counterparts and typically require a large amount of instrument time for low concentration samples. In this study, we demonstrate how the sensitivity limitation may be overcome by transiently increasing the bulk polarization using signal amplification by reversible exchange (SABRE) hyperpolarization. We utilize para-enriched dihydrogen to enhance the pure shift NMR resonances of pyridine by up to a factor of 60 in a single-scan experiment and extend this to propose a method to unambiguously determine mixture components based on the enhancement of their pure shift NMR signals.

中文翻译:

SABRE 增强的实时纯位移 NMR 光谱

纯位移核磁共振 (NMR) 方法抑制同核标量耦合的影响,以产生仅由每个化学不同位点的单个信号组成的 NMR 光谱。它们越来越多地用于分析复杂分子和混合物,因为它们克服了使除最简单物质之外的所有质子 NMR 光谱复杂化的广泛信号重叠。与传统方法相比,目前用于 1D 质子光谱的宽带纯位移方法的灵敏度降低,并且对于低浓度样品通常需要大量的仪器时间。在这项研究中,我们展示了如何通过使用可逆交换 (SABRE) 超极化的信号放大瞬时增加体极化来克服灵敏度限制。
更新日期:2021-08-17
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