Preimplantation embryo development is characterized by drastic nuclear reprogramming and dynamic stage-specific gene expression. Key regulators of this earliest developmental stage have not been revealed. In the present study, a “non-classical” nuclear-localization pattern of eIF1A was observed during early developmental stages of mouse preimplantation embryo before late-morula. In particular, eIF1A is most highly expressed in the nuclear of 2-cell embryo. Knockdown eIF1A by siRNA microinjection affected the development of mouse preimplantation embryo, resulted in decreased blastocyst formation rate. CDX2 protein expression level significantly down-regulated after eIF1A knockdown in morula stage. In addition, the mRNA expression level of Hsp70.1 was also decreased in 2-cell embryo. The results indicate an indispensable role of eIF1A in mouse preimplantation embryos.

植入前胚胎发育的特点是剧烈的核重编程和动态的阶段特异性基因表达。这个最早发展阶段的关键调节器尚未被发现。在本研究中，在晚期桑椹前小鼠植入前胚胎的早期发育阶段观察到了 eIF1A 的“非经典”核定位模式。特别是，eIF1A 在 2 细胞胚胎的细胞核中表达最高。siRNA显微注射敲低eIF1A影响小鼠着床前胚胎的发育，导致囊胚形成率降低。在桑椹胚期 eIF1A 敲低后 CDX2 蛋白表达水平显着下调。此外，2细胞胚胎中Hsp70.1的mRNA表达水平也降低。