ABSTRACT

This study examined the associations between PM (2.5 and 10) and global DNA methylation among 100 e-waste workers and 51 non-e-waste workers serving as controls. Long interspersed nucleotide repetitive elements-1 (LINE-1) was measured by pyrosequencing. Personal PM_2.5 and PM₁₀ were measured over a 4-hour work-shift using real-time particulate monitors incorporated into a backpack . Linear regression models were used to assess the association between PM and LINE-1 DNA methylation. The concentrations of PM_2.5 and PM₁₀ were significantly higher among the e-waste workers than the controls (77.32 vs 34.88, p < 0.001 and 210.21 vs 121.92, p < 0.001, respectively). PM_2.5 exposure was associated with increased LINE-1 CpG2 DNA methylation (β = 0.003; 95% CI; 0.001, 0.006; p = 0.022) but not with the average of all 4 CpG sites of LINE-1. In summary, high levels of PM_2.5 exposure was associated with increased levels of global DNA methylation in a site-specific manner.

摘要

本研究检查了作为对照的 100 名电子废物工人和 51 名非电子废物工人中 PM（2.5 和 10）与全球 DNA 甲基化之间的关联。通过焦磷酸测序测量长散布的核苷酸重复元件-1 (LINE-1)。个人 PM _2.5和 PM ₁₀是在 4 小时的轮班工作中使用集成在背包中的实时颗粒物监测器测量的。线性回归模型用于评估 PM 和 LINE-1 DNA 甲基化之间的关联。_{电子垃圾处理人员中 PM 2.5}和 PM ₁₀的浓度显着高于对照组（分别为 77.32 vs 34.88，p < 0.001和 210.21 vs 121.92，p < 0.001）。下午_2.5暴露与 LINE-1 CpG2 DNA 甲基化增加有关（β = 0.003；95% CI；0.001, 0.006；p = 0.022），但与 LINE-1 的所有 4 个 CpG 位点的平均值无关。总之，高水平的 PM _2.5暴露与位点特异性方式的全球 DNA 甲基化水平增加有关。