Purpose: Hydrogen (H₂) is an antioxidant with anti-inflammatory and apoptosis functions.This study aimed to estimate the effects of H₂ on acute myocardial infarction (AMI) in rats and its association with the inhibition of oxidative stress and cardiomyocyte pyroptosis./nMethods: Sixty-four rats were randomly divided into three groups (Sham, AMI, and H₂). The left anterior descending coronary artery (LAD) of rats in the AMI and H₂ groups was ligated, while rats in the Sham group were threaded without ligation. In addition, 2% H₂ was administered by inhalation for 24 h after ligation in the H₂ group. Transthoracic echocardiography was performed after H₂ inhalation, followed by collection of the serum and cardiac tissue of all rats./nResults: H₂ inhalation ameliorated the cardiac dysfunction, infarct size and inflammatory cell infiltration caused by AMI. Meanwhile, H₂ inhalation reduced the concentration of serum Troponin I (TnI), brain natriuretic peptide (BNP), reactive oxygen species (ROS), cardiac malondialdehyde (MDA), and 8-OHdG. In addition, H₂ inhalation inhibited cardiac inflammation and pyroptosis relative proteins expression./nConclusion: H₂ effectively promoted heart functions in AMI rats by regulating oxidative stress and pyroptosis.

中文翻译：

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氢气通过调节氧化应激和 NLRP3 炎性体介导的细胞焦亡减轻大鼠心肌损伤

目的：氢（H ₂）是一种具有抗炎和凋亡功能的抗氧化剂。本研究旨在评估H ₂对大鼠急性心肌梗死（AMI）的影响及其与抑制氧化应激和心肌细胞焦亡的关系。 /n方法： 64只大鼠随机分为三组（Sham、AMI和H ₂）。AMI组和H ₂组大鼠左冠状动脉前降支(LAD)结扎，Sham组大鼠不结扎穿线。此外，2％H ₂通过吸入24小时结扎后在H施用₂团体。吸入H ₂后进行经胸超声心动图检查，收集所有大鼠的血清和心脏组织。/n结果：吸入H _{2 可}改善AMI引起的心功能障碍、梗死面积和炎性细胞浸润。同时，H ₂吸入降低了血清肌钙蛋白 I (TnI)、脑利钠肽 (BNP)、活性氧 (ROS)、心脏丙二醛 (MDA) 和 8-OHdG 的浓度。此外，吸入H ₂可抑制心脏炎症和细胞焦亡相关蛋白的表达。/n结论： H _{2 可}通过调节氧化应激和细胞焦亡有效促进AMI大鼠心脏功能。