Cholesteatoma of the middle ear is a common disease in otolaryngology, which can lead to serious intracranial and extracranial complications. Recent studies showed that the dysregulation of microRNA may be involved in the formation of middle ear cholesteatoma. This study aimed to explore the regulatory effect of micro ribonucleic acid 508-3p (miR-508-3p) on proliferation and apoptosis of middle ear cholesteatoma cells and excavate its underlying regulatory mechanism. We found miR-508-3p expression was upregulated in tissues and cells of cholesteatoma which was inversely related to the expression of hsa_circ_0000007. Overexpression of miR-508-3p could notably facilitate cholesteatoma cell proliferation. Luciferase reporter assay showed that miR-508-3p bound the 3'-untranslated region of its downstream mRNA PTEN. Gain and loss of functions of miR-508-3p were performed to identify their roles in the biological behaviors of cholesteatoma cells, including proliferation and apoptosis. Rescue assays confirmed that PTEN could reverse the effect of miR-508-3p overexpression on cell proliferation. In a word, this study validated that the development of cholesteatoma may regulated by hsa_circ_0000007/miR-508-3p/ PTEN/ PI3K/Akt axis.

中文翻译：

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MiR-508-3p通过靶向PTEN/PI3K/AKT通路促进中耳胆脂瘤细胞增殖并抑制凋亡

中耳胆脂瘤是耳鼻喉科的常见病，可导致严重的颅内外并发症。最近的研究表明，microRNA的失调可能与中耳胆脂瘤的形成有关。本研究旨在探讨微核糖核酸508-3p（miR-508-3p）对中耳胆脂瘤细胞增殖和凋亡的调控作用，并挖掘其潜在调控机制。我们发现miR-508-3p在胆脂瘤组织和细胞中的表达上调，这与hsa_circ_0000007的表达呈负相关。miR-508-3p的过表达可以显着促进胆脂瘤细胞增殖。荧光素酶报告基因检测显示miR-508-3p结合其下游 mRNA PTEN的 3'-非翻译区。进行了miR-508-3p功能的获得和丧失，以确定它们在胆脂瘤细胞的生物学行为中的作用，包括增殖和凋亡。救援试验证实 PTEN 可以逆转miR-508-3p过表达对细胞增殖的影响。总之，本研究验证了胆脂瘤的发展可能受hsa_circ_0000007 / miR-508-3p /PTEN/PI3K/Akt轴调控。