Abstract

Based on high-performance liquid chromatography-mass spectrometry (HPLC-MS) and reversed-phase high-performance liquid chromatography-mass spectrometry (RPLC-MS), the changes of drug metabolism and endogenous metabolites in normal rats and tumor model rats were studied by drug metabonomics. The changes of vital signs were used to evaluate the toxicity and pharmacological effects of the new anti-cancer candidate ST on rats, and the differences of endogenous metabolites caused by ST were analyzed by multivariate statistical method. The changes of endogenous metabolites related to ST toxicity and pharmacological effects were analyzed. Studies have shown that the correlation analysis of metabonomics based on metabonomics and changes of animal signs is helpful to screen endogenous metabolites related to drug toxicity and pharmacological effects.

摘要

基于高效液相色谱-质谱（HPLC-MS）和反相高效液相色谱-质谱（RPLC-MS），研究正常大鼠和肿瘤模型大鼠药物代谢和内源性代谢物的变化。通过药物代谢组学。采用生命体征变化评价新型抗癌候选ST对大鼠的毒性和药理作用，采用多元统计方法分析ST引起的内源性代谢产物的差异。分析与ST毒性和药理作用相关的内源性代谢产物的变化。研究表明，基于代谢组学与动物体征变化的代谢组学相关性分析，有助于筛选与药物毒性和药理作用相关的内源性代谢物。