Agriculture Sector has been affected largely by biotic and abiotic stresses. Among biotic challenges faced by agriculture is the damage caused by insect pests. Insecticides are being widely used for rapid and effective management of insect pests. Despite of their usefulness, the excessive use of insecticides poses potential risks to food safety and environment. To reduce the usage of chemicals, biological control of insect pest can be effective alternative of insecticides for sustainable agriculture. The present study aims to evaluate the insecticidal potential of a protein obtained from an endophytic Aspergillus awamori against insect pest Spodoptera litura (Fab). Variable concentrations of the inhibitor induced high larval mortality ranging from 16.67 to 53%. Larval, pupal and total developmental period of S. litura increased significantly at higher concentrations. The LC₅₀ value of the inhibitor was determined to be 34.34 μg/ml. A significant decrease in adult emergence was recorded at all the concentrations with maximum inhibitory effect at 30 μg/ml where only 65.94% adults emerged as compared to 100.00% in control. The in vivo effect of the inhibitor on gut α-amylase and α-glucosidase was also studied. The activity of α-amylase and α-glucosidase was suppressed to 33.84% and 38.13%, respectively after 72 h at highest concentration of 40 μg/ml. The biosafety evaluation of the extract revealed that the strain is ochratoxin negative and noncytotoxic which supports the above findings that it could be used as biocontrol agent.

农业部门主要受到生物和非生物胁迫的影响。农业面临的生物挑战之一是虫害造成的破坏。杀虫剂正被广泛用于快速有效地防治害虫。尽管杀虫剂有用，但过度使用杀虫剂对食品安全和环境构成潜在风险。为了减少化学品的使用，昆虫害虫的生物防治可以成为可持续农业杀虫剂的有效替代品。本研究旨在评估从内生泡盛曲霉中获得的蛋白质对昆虫害虫斜纹夜蛾的杀虫潜力(Fab)。不同浓度的抑制剂诱导高幼虫死亡率，范围从 16.67% 到 53%。幼虫，蛹和总发育期间斜纹夜蛾在较高浓度显著增加。抑制剂的LC ₅₀值确定为34.34 μg/ml。在所有浓度下都记录到成虫出现显着减少，在 30 μg/ml 时具有最大抑制作用，与​​对照中的 100.00% 相比，只有 65.94% 的成虫出现。在体内还研究了抑制剂对肠道α-淀粉酶和α-葡萄糖苷酶的影响。在 40 μg/ml 的最高浓度下，72 小时后，α-淀粉酶和α-葡萄糖苷酶的活性分别被抑制到 33.84% 和 38.13%。提取物的生物安全性评估表明，该菌株是赭曲霉毒素阴性且无细胞毒性，这支持了上述发现，即它可以用作生物防治剂。