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Potential role of folic acid in preventing male infertility associated with MTHFR gene C677T (rs1801133) polymorphism
Frontiers in Life Science ( IF 1.333 ) Pub Date : 2021-08-17 , DOI: 10.1080/26895293.2021.1963846
Fahad A. Alhumaydhi 1 , Amal M. H. Mackawy 1, 2 , Enas N. Morgan 3, 4 , Waleed Al Abdulmonem 5 , Suliman A. Alsagaby 6 , Ameen S. S. Alwashmi 1 , Abdullah S. M. Aljohani 7 , Mohammad A. Aljasir 1 , Saleh A. Almatroodi 1 , Abdulmohsen M. Alruwetei 1 , Ayman M. Mousa 8, 9
Affiliation  

Folic acid and methylenetetrahydrofolate reductase enzyme (MTHFR) gene C677T (rs1801133) SNP have been proofed to influence the spermatogenesis. This study aimed to investigate the biochemical, histological, and immunohistochemical (IHC) of adult male rabbits after induction of infertility by adenine, and the role of MTHFR C677T genotypes with or without folic acid therapy. Thirty adult male rabbits were divided into three groups: control (G1), adenine-induced infertility (G2), and adenine-induced infertility with folic acid therapy (G3). The PCR-RFLP was applied to detect the MTHFR C677T genotypes. Biochemical analyses of seminal fluid, homocysteine, and sex hormones were determined with histological and IHC examination of testicular tissue. Morphometric measurements and a TUNEL assay of seminiferous tubules were also performed. The results revealed a significant increase in homocysteine and sperm abnormalities, while sex hormones, sperm count and motility decreased significantly in the TT genotype compared with the CC genotype. Also, G2 displayed a significant increase in homocysteine and sperm abnormalities, with a significant decrease in sex hormones, sperm count and motility as compared to the G1 and G3. In conclusion, MTHFR 677C > T polymorphism is a genetic risk factor for male infertility and folic acid has a potential role in the treatment of male infertility.



中文翻译:

叶酸在预防与 MTHFR 基因 C677T (rs1801133) 多态性相关的男性不育症中的潜在作用

叶酸和亚甲基四氢叶酸还原酶 (MTHFR) 基因 C677T (rs1801133) SNP 已被证明会影响精子发生。本研究旨在调查成年雄兔在腺嘌呤诱导不孕后的生化、组织学和免疫组织化学 (IHC),以及 MTHFR C677T 基因型在有或没有叶酸治疗的情况下的作用。三十只成年雄性兔被分为三组:对照组(G1)、腺嘌呤诱导的不孕症(G2)和叶酸治疗的腺嘌呤诱导的不孕症(G3)。PCR-RFLP 用于检测 MTHFR C677T 基因型。通过睾丸组织的组织学和 IHC 检查确定精液、同型半胱氨酸和性激素的生化分析。还进行了生精小管的形态测量和 TUNEL 测定。结果显示,与CC基因型相比,TT基因型的同型半胱氨酸和精子异常显着增加,而性激素、精子数量和活力显着降低。此外,与 G1 和 G3 相比,G2 显示出同型半胱氨酸和精子异常的显着增加,性激素、精子数量和运动能力显着下降。总之,MTHFR 677C > T 多态性是男性不育的遗传危险因素,叶酸在治疗男性不育中具有潜在作用。与 G1 和 G3 相比,性激素、精子数量和活力显着降低。总之,MTHFR 677C > T 多态性是男性不育的遗传危险因素,叶酸在治疗男性不育中具有潜在作用。与 G1 和 G3 相比,性激素、精子数量和活力显着降低。总之,MTHFR 677C > T 多态性是男性不育的遗传危险因素,叶酸在治疗男性不育中具有潜在作用。

更新日期:2021-08-17
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