当前位置: X-MOL 学术Acta Trop. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Toxic effect of Croton rudolphianus leaf essential oil against Biomphalaria glabrata, Schistosoma mansoni cercariae and Artemia salina
Acta Tropica ( IF 2.7 ) Pub Date : 2021-08-17 , DOI: 10.1016/j.actatropica.2021.106102
Ingridd Ayslane T A Ribeiro 1 , José Luiz F Sá 1 , Maíra V Lima 2 , Shyrlane T S Veras 3 , Júlio César R O F Aguiar 4 , André L Aires 5 , Mônica C P A Albuquerque 5 , Márcia Vanusa da Silva 1 , Ana Maria M A Melo 2 , Daniela Maria A F Navarro 4 , Maria Tereza S Correia 1
Affiliation  

This research investigated the effect of the Croton rudolphianus leaf essential oil (EO) on Biomphalaria glabrata embryos (at different development stages) and adults, Schistosoma mansoni cercariae, and Artemia salina (non-target organism). It was possible to identify 31 compounds in the C. rudolphianus EO through GC-MS analysis. The major compounds from this oil were (E)-caryophyllene (17.33%), an unknown compound (16.87%), bicyclogermacrene (7.1%), δ-cadinene (6.62%) and germacrene D (5.38%). After incubation for 24 h, the EO of C. rudolphianus induced the occurrence of non-viable embryos (dead and malformed), with an LC50 value of 126.54, 133.51, 143.53 and 161.95 µg/mL and an LC90 value of 202.61, 216.48, 232.98 and 271.16 µg/mL to blastula, gastrula, trochophore and veliger embryonic stages, respectively. The EO was more effective against B. glabrata adults (LC50 and LC90 = 47.89 and 78.86 µg/mL, respectively), and S. mansoni cercariae (LC50 and LC90 = 14.81 and 22.15 after 120 mins of exposure, respectively) than against B. glabrata embryos. Concerning the micronucleus assay, the mean frequency of apoptosis, binucleation and micronucleus were 45.33 ± 3.51, 19.33 ± 1.53 and 0.67 ± 0.58 per 1000 cells at 25 µg/mL, which is the highest concentration tested. The oil killed A. salina with LC50 and LC90 values (68.33 and 111.5 µg/mL, respectively) higher than those determined for adult snails and S. mansoni cercariae. In conclusion, C. rudolphianus EO had a toxic effect against B. glabrata adults and embryos, and S. mansoni cercariae. Furthermore, this oil showed to be cytotoxic to hemocytes of B. glabrata. Concerning the non-target organism assay, C. rudolphianus EO was less toxic to A. salina then to adult snails and S. mansoni cercariae. Due to this, the EO from C. rudolphianus leaves is a potential alternative for schistosomiasis control.



中文翻译:

巴豆叶精油对光斑蛔虫、曼氏血吸虫尾蚴和卤虫的毒害作用

这项研究调查了效果巴豆rudolphianus叶精油(EO)贝罗光滑的胚胎(在不同的发展阶段)和成人,曼氏血吸虫尾蚴,并卤虫(非靶标生物)。通过 GC-MS 分析,可以在C. rudolphianus EO 中鉴定出 31 种化合物。该油中的主要化合物是 ( E )-石竹烯 (17.33%)、未知化合物 (16.87%)、双环锗烯 (7.1%)、δ-卡二烯 (6.62%) 和锗烯 D (5.38%)。孵育 24 小时后,C. rudolphianus的 EO诱导了非存活胚胎(死亡和畸形)的发生,LC 50126.54、133.51、143.53 和 161.95 µg/mL 的值以及 202.61、216.48、232.98和 271.16 µg/mL 的 LC 90值分别对囊胚、原肠胚、胚泡和胚胎阶段。EO 对B. glabrata成虫(LC 50和 LC 90 分别 为 47.89 和 78.86 µg/mL)和曼氏尾蚴(LC 50和 LC 90 在暴露 120 分钟后分别为 14.81 和 22.15)更有效比对抗B. glabrata胚胎。关于微核测定,在 25 µg/mL 浓度下,每 1000 个细胞凋亡、双核和微核的平均频率分别为 45.33 ± 3.51、19.33 ± 1.53 和 0.67 ± 0.58,这是测试的最高浓度。该油杀死了A. salina,其 LC 50和 LC 90值(分别为 68.33 和 111.5 µg/mL)高于对成年蜗牛和S. mansoni尾蚴的测定值。总之,C. rudolphianus EO 对B. glabrata成虫和胚胎以及S. mansoni尾蚴具有毒性作用。此外,该油显示对光滑双歧杆菌的血细胞具有细胞毒性。关于非靶标生物测定,C. rudolphianus EO 对A. salina 的毒性低于对成年蜗牛和S. mansoni尾蚴的毒性。因此,来自C. rudolphianus叶的 EO是控制血吸虫病的潜在替代品。

更新日期:2021-08-30
down
wechat
bug