Acta Tropica ( IF 2.7 ) Pub Date : 2021-08-17 , DOI: 10.1016/j.actatropica.2021.106102 Ingridd Ayslane T A Ribeiro 1 , José Luiz F Sá 1 , Maíra V Lima 2 , Shyrlane T S Veras 3 , Júlio César R O F Aguiar 4 , André L Aires 5 , Mônica C P A Albuquerque 5 , Márcia Vanusa da Silva 1 , Ana Maria M A Melo 2 , Daniela Maria A F Navarro 4 , Maria Tereza S Correia 1
This research investigated the effect of the Croton rudolphianus leaf essential oil (EO) on Biomphalaria glabrata embryos (at different development stages) and adults, Schistosoma mansoni cercariae, and Artemia salina (non-target organism). It was possible to identify 31 compounds in the C. rudolphianus EO through GC-MS analysis. The major compounds from this oil were (E)-caryophyllene (17.33%), an unknown compound (16.87%), bicyclogermacrene (7.1%), δ-cadinene (6.62%) and germacrene D (5.38%). After incubation for 24 h, the EO of C. rudolphianus induced the occurrence of non-viable embryos (dead and malformed), with an LC50 value of 126.54, 133.51, 143.53 and 161.95 µg/mL and an LC90 value of 202.61, 216.48, 232.98 and 271.16 µg/mL to blastula, gastrula, trochophore and veliger embryonic stages, respectively. The EO was more effective against B. glabrata adults (LC50 and LC90 = 47.89 and 78.86 µg/mL, respectively), and S. mansoni cercariae (LC50 and LC90 = 14.81 and 22.15 after 120 mins of exposure, respectively) than against B. glabrata embryos. Concerning the micronucleus assay, the mean frequency of apoptosis, binucleation and micronucleus were 45.33 ± 3.51, 19.33 ± 1.53 and 0.67 ± 0.58 per 1000 cells at 25 µg/mL, which is the highest concentration tested. The oil killed A. salina with LC50 and LC90 values (68.33 and 111.5 µg/mL, respectively) higher than those determined for adult snails and S. mansoni cercariae. In conclusion, C. rudolphianus EO had a toxic effect against B. glabrata adults and embryos, and S. mansoni cercariae. Furthermore, this oil showed to be cytotoxic to hemocytes of B. glabrata. Concerning the non-target organism assay, C. rudolphianus EO was less toxic to A. salina then to adult snails and S. mansoni cercariae. Due to this, the EO from C. rudolphianus leaves is a potential alternative for schistosomiasis control.
中文翻译:
巴豆叶精油对光斑蛔虫、曼氏血吸虫尾蚴和卤虫的毒害作用
这项研究调查了效果巴豆rudolphianus叶精油(EO)贝罗光滑的胚胎(在不同的发展阶段)和成人,曼氏血吸虫尾蚴,并卤虫(非靶标生物)。通过 GC-MS 分析,可以在C. rudolphianus EO 中鉴定出 31 种化合物。该油中的主要化合物是 ( E )-石竹烯 (17.33%)、未知化合物 (16.87%)、双环锗烯 (7.1%)、δ-卡二烯 (6.62%) 和锗烯 D (5.38%)。孵育 24 小时后,C. rudolphianus的 EO诱导了非存活胚胎(死亡和畸形)的发生,LC 50126.54、133.51、143.53 和 161.95 µg/mL 的值以及 202.61、216.48、232.98和 271.16 µg/mL 的 LC 90值分别对囊胚、原肠胚、胚泡和胚胎阶段。EO 对B. glabrata成虫(LC 50和 LC 90 分别 为 47.89 和 78.86 µg/mL)和曼氏尾蚴(LC 50和 LC 90 在暴露 120 分钟后分别为 14.81 和 22.15)更有效比对抗B. glabrata胚胎。关于微核测定,在 25 µg/mL 浓度下,每 1000 个细胞凋亡、双核和微核的平均频率分别为 45.33 ± 3.51、19.33 ± 1.53 和 0.67 ± 0.58,这是测试的最高浓度。该油杀死了A. salina,其 LC 50和 LC 90值(分别为 68.33 和 111.5 µg/mL)高于对成年蜗牛和S. mansoni尾蚴的测定值。总之,C. rudolphianus EO 对B. glabrata成虫和胚胎以及S. mansoni尾蚴具有毒性作用。此外,该油显示对光滑双歧杆菌的血细胞具有细胞毒性。关于非靶标生物测定,C. rudolphianus EO 对A. salina 的毒性低于对成年蜗牛和S. mansoni尾蚴的毒性。因此,来自C. rudolphianus叶的 EO是控制血吸虫病的潜在替代品。