Purpose

To develop an in vitro culture system for tissue engineering to mimic the in vivo environment and evaluate the applicability of ultrasound and PLGA particle system.

Methods

For tissue engineering, large molecules such as growth factors for cell differentiation should be supplied in a controlled manner into the culture system, and the in vivo microenvironment need to be reproduced in the system for the regulation of cellular function. In this study, portable prototype ultrasound with low intensity was devised and tested for protein release from bovine serum albumin (BSA)-loaded poly(lactic-co-glycolic acid) (PLGA) particles.

Results

BSA-loaded PLGA particles were prepared using various types of PLGA reagents and their physicochemical properties were characterized including particle size, shape, or aqueous wetting profiles. The BSA-loaded formulation showed nano-ranged size distribution with optimal physical stability during storage period, and protein release behaviors in a controlled manner. Notably, the application of prototype ultrasound with low intensity influenced protein release patterns in the culture system containing the BSA-loaded PLGA formulation. The results revealed that the portable ultrasound set controlled by the computer could contribute for the protein delivery in the culture medium.

Conclusions

This study suggests that combined application with ultrasound and protein-loaded PLGA encapsulation system could be utilized to improve culture system for tissue engineering or cell regeneration therapy.

中文翻译：

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原型超声和加载 BSA 的 PLGA 颗粒在蛋白质递送中的联合应用

目的

开发用于组织工程的体外培养系统，以模拟体内环境并评估超声和 PLGA 粒子系统的适用性。

方法

对于组织工程而言，细胞分化的生长因子等大分子需要以可控的方式供应到培养系统中，并且需要在系统中再现体内微环境以调节细胞功能。在这项研究中，设计了具有低强度的便携式原型超声，并测试了载有牛血清白蛋白 (BSA) 的聚 (乳酸-共-乙醇酸) (PLGA) 颗粒的蛋白质释放。

结果

使用各种类型的 PLGA 试剂制备负载 BSA 的 PLGA 颗粒，并对其物理化学性质进行表征，包括颗粒大小、形状或水润湿特性。负载 BSA 的配方显示出纳米级尺寸分布，在储存期间具有最佳的物理稳定性，并以受控方式释放蛋白质。值得注意的是，低强度原型超声的应用影响了含有加载 BSA 的 PLGA 制剂的培养系统中的蛋白质释放模式。结果表明，由计算机控制的便携式超声装置有助于培养基中的蛋白质输送。

结论

本研究表明，超声和蛋白质负载 PLGA 封装系统的联合应用可用于改进组织工程或细胞再生治疗的培养系统。