Adult human multipotent neural cells (ahMNCs) are unique cells derived from adult human temporal lobes. They show multipotent differentiation potentials into neurons and astrocytes. In addition, they possess proangiogenic capacities. The objective of this study was to characterize ahMNCs in terms of expression of cell type-specific markers, in vitro differentiation potentials, and paracrine factors compared with several other cell types including fetal neural stem cells (fNSCs) to provide detailed molecular and functional features of ahMNCs. Interestingly, the expression of cell type-specific markers of ahMNCs could not be differentiated from those of pericytes, mesenchymal stem cells (MSCs), or fNSCs. In contrast, differentiation potentials of ahMNCs and fNSCs into neural cells were higher than those of other cell types. Compared with MSCs, ahMNCs showed lower differentiation capacities into osteogenic and adipogenic cells. Moreover, ahMNCs uniquely expressed higher levels of MCP-1 and GRO family paracrine factors than fNSCs and MSCs. These high levels of MCP-1 and GRO family mediated in vivo proangiogenic effects of ahMNCs. These results indicate that ahMNCs have their own distinct characteristics that could distinguish ahMNCs from other cell types. Characteristics of ahMNCs could be utilized further in the preclinical and clinical development of ahMNCs for regenerative medicine. They could also be used as experimental references for other cell types including fNSCs.

中文翻译：

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成人多能神经细胞可以通过 MCP-1 和 GRO 的促血管生成旁分泌作用与其他细胞类型区分开来

成人多能神经细胞 (ahMNC) 是源自成人颞叶的独特细胞。它们显示出向神经元和星形胶质细胞的多能分化潜能。此外，它们具有促血管生成能力。本研究的目的是根据细胞类型特异性标志物的表达来表征 ahMNCs，体外分化潜能和旁分泌因子与包括胎儿神经干细胞 (fNSC) 在内的几种其他细胞类型相比，以提供 ahMNC 的详细分子和功能特征。有趣的是，ahMNCs 的细胞类型特异性标志物的表达无法与周细胞、间充质干细胞 (MSCs) 或 fNSCs 的那些标志物区分开来。相比之下，ahMNCs 和 fNSCs 向神经细胞的分化潜能高于其他细胞类型。与 MSCs 相比，ahMNCs 向成骨细胞和脂肪细胞的分化能力较低。此外，ahMNCs 比 fNSCs 和 MSCs 独特地表达更高水平的 MCP-1 和 GRO 家族旁分泌因子。这些高水平的 MCP-1 和 GRO 家族在体内介导ahMNCs 的促血管生成作用。这些结果表明 ahMNCs 具有自己独特的特征，可以将 ahMNCs 与其他细胞类型区分开来。ahMNCs 的特性可以进一步用于 ahMNCs 用于再生医学的临床前和临床开发。它们还可用作其他细胞类型（包括 fNSC）的实验参考。