Pimenta dioica (L.) Merr. is a popular seasoning species. During its production, there are losses of up to 50% due to anthracnose caused by fungi of the genus Colletotrichum. In this study, we evaluated the effect of ethanolic extracts on the in vitro and in vivo control of isolated Colletotrichum spp. Allspice. Extracts of leaves of P. dioica, Piper auritum, Plectranthus amboinicus and Psidium guajava were chemically characterized by gas chromatography and high performance liquid chromatography. Several monoterpenes, sesquiterpenes, flavonoids, organic acids, alkaloids and monoterpenoid phenols were identified among other organic compounds. Alpha-ocimene in P. dioica; alpha-thujene and isosafrole in P. auritum; kumatakenin, cirsilineol, taxifolin and retusin in P. amboinicus are reported for the first time. The extracts of P. amboinicus and P. dioica inhibited mycelial growth, spore germination in Colletotrichum spp. at concentrations of 5, 10 and 20 mg/mL, while the extracts of P. guajava and P. auritum did so at concentrations of 30 and 40 mg/mL. The extracts of P. amboinicus, P. dioica and P. auritum modified the size of the spores, caused degradation and formation of vacuoles in the spores of C. acutatum and C. gloeosporioides. The incidences in fruit inoculated with C. boninense and treated with the extracts of P. amboinicus and P. dioica were of 12% and 4% respectively. The severity was 1.5% with the extract of P. amboinicus and 0% with P. dioica. The results show the richness of bioactive compounds present in the extracts and their possible application to develop biotechnological products against phytopathogenic fungi.

Pimenta dioica (L.) Merr. 是一种流行的调味品。在其生产过程中，由于炭疽菌属真菌引起的炭疽病，损失高达 50% 。在这项研究中，我们评估了乙醇提取物对分离炭疽菌的体外和体内控制的影响。五香粉。P. dioica、Piper auritum、Plectranthus amboinicus和Psidium guajava的叶子提取物用气相色谱和高效液相色谱进行化学表征。在其他有机化合物中鉴定出几种单萜、倍半萜、黄酮类、有机酸、生物碱和单萜酚。P. dioica 中的α-罗勒烯；P. auritum 中的alpha-thujene 和异黄樟素；首次报道了P. amboinicus中的kumatakenin、cirsilineol、taxfolin 和 retusin 。P. amboinicus和P. dioica的提取物抑制了炭疽菌中的菌丝体生长和孢子萌发。浓度为 5、10 和 20 mg/mL，而P. guajava和P. auritum的提取物在 30 和 40 毫克/毫升的浓度下这样做。P. amboinicus、P. dioica和P. auritum的提取物改变了孢子的大小，导致C. acutatum和C. gloeosporioides的孢子降解和形成液泡。接种了C. boninense并用P. amboinicus和P. dioica提取物处理的果实中的发生率分别为12% 和4%。P. amboinicus提取物的严重程度为 1.5%，P . dioica 为0% 。 结果表明，提取物中含有丰富的生物活性化合物，以及它们在开发针对植物病原真菌的生物技术产品中的可能应用。