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Tissue-specific smooth muscle cell subtypes identified by transcriptional profiling
The International Journal of Biochemistry & Cell Biology ( IF 4 ) Pub Date : 2021-08-16 , DOI: 10.1016/j.biocel.2021.106055
Chien-Jung Lin 1 , Robert P Mecham 2
Affiliation  

Smooth muscle cells (SMCs) are specialized cells present in many organs where they serve diverse tissue-specific functions. Using the Tabula Muris compendium of single-cell RNA sequencing data, we extracted individual SMC transcriptomes from eight mouse tissues to investigate the transcriptomic landscape of tissue-specific SMCs. We identified marker genes, signaling pathways, and biological processes enriched in tissue-specific SMCs, and inferred potential ligand-receptor interaction between SMC and other cell types. Our analysis also identified sex differences in SMC gene expression in different tissues. Lastly, we used unsupervised clustering to identify novel SMC subtypes based on their downstream targets of transcription factors. Our results highlight the variable SMC phenotypes and underscore this cell’s remarkable adaptability to contribute to diverse tissue function.



中文翻译:

通过转录谱鉴定的组织特异性平滑肌细胞亚型

平滑肌细胞 (SMC) 是存在于许多器官中的特殊细胞,它们在其中发挥多种组织特异性功能。使用 Tabula Muris 单细胞 RNA 测序数据纲要,我们从 8 种小鼠组织中提取了单个 SMC 转录组,以研究组织特异性 SMC 的转录组学景观。我们鉴定了富含组织特异性 SMC 的标记基因、信号通路和生物学过程,并推断出 SMC 与其他细胞类型之间潜在的配体-受体相互作用。我们的分析还确定了不同组织中 SMC 基因表达的性别差异。最后,我们使用无监督聚类根据转录因子的下游靶标来识别新的 SMC 亚型。

更新日期:2021-08-20
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