Abstract

Surface treatments are designed to promote modified implant surfaces with positive interactions with the surrounding living tissues. However, the inadvertent early contact of these surfaces with oral fluids during surgery may lead to undesired conditions affecting osseointegration. This study aimed to investigate the possible alterations in the physico-chemical properties of modified-surfaces caused by early saliva exposure. Titanium (Ti) surfaces were exposed to three different samples of human saliva and later analyzed for protein adhesion, physico-chemical surface alterations, and osteogenic cell-viability. The results indicated that surface roughness was the most significant factor influencing saliva protein adsorption; moreover, hydrophilic surfaces had critically lost their characteristics after contact with saliva. Decreased cell viability was observed in cultures after contact with saliva. Early contact with saliva might negatively influence modified surface properties and local cell viability. Careful surgical insertion of implants with hydrophilic surfaces is recommended, particularly in sites where saliva interaction is prone to occur.

摘要

表面处理旨在促进改良的种植体表面与周围活组织的积极相互作用。然而，这些表面在手术过程中与口腔液体的早期接触可能会导致影响骨整合的不良情况。本研究旨在调查由早期唾液暴露引起的改性表面物理化学性质的可能变化。钛 (Ti) 表面暴露于三种不同的人类唾液样本，随后分析了蛋白质粘附、物理化学表面改变和成骨细胞活力。结果表明，表面粗糙度是影响唾液蛋白吸附的最显着因素；此外，亲水表面在与唾液接触后严重失去了它们的特性。在与唾液接触后的培养物中观察到细胞活力降低。早期接触唾液可能会对改性表面特性和局部细胞活力产生负面影响。建议小心地通过手术插入具有亲水表面的植入物，特别是在容易发生唾液相互作用的部位。