We have developed a low-cost, chromatic confocal endomicroscope (CCE) that can image a cross-section of the tissue at cellular resolution. In CCE, a custom miniature objective lens was used to focus different wavelengths into different tissue depths. Therefore, each tissue depth was encoded with the wavelength. A custom miniature spectrometer was used to spectrally-disperse light reflected from the tissue and generate cross-sectional confocal images. The CCE prototype had a diameter of 9.5 mm and a length of 68 mm. Measured resolution was high, 2 µm and 4 µm for lateral and axial directions, respectively. Effective field size was 468 µm. Preliminary results showed that CCE can visualize cellular details from cross-sections of the tissue in vivo down to the tissue depth of 100 µm.

中文翻译：

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用于体内细胞成像的低成本、彩色共聚焦内窥镜

我们开发了一种低成本的彩色共聚焦内窥镜 (CCE)，可以以细胞分辨率对组织的横截面进行成像。在 CCE 中，使用定制的微型物镜将不同的波长聚焦到不同的组织深度。因此，每个组织深度都用波长编码。使用定制的微型光谱仪对从组织反射的光进行光谱色散并生成横截面共焦图像。CCE 原型的直径为 9.5 毫米，长度为 68 毫米。测得的分辨率很高，横向和轴向方向分别为 2 µm 和 4 µm。有效视野大小为 468 µm。初步结果表明，CCE 可以可视化从体内组织横截面到 100 µm 组织深度的细胞细节。