The aim of the present study was to quantify selected phenolic compounds, determine antioxidant activity and enzyme inhibitory effects of the aerial parts of Alkanna trichophylla Hub.-Mor. (A. trichophylla) and Convolvulus galaticus Rost.ex Choisy (C. galaticus) extracts prepared by homogenizer-assisted extraction (HAE), maceration (MAC) and infusion techniques. This is the first time such study has been designed to validate the phytochemical composition and bioactivity of these plants. Multivariate analysis was conducted on collected data. Rutin and caffeoylquinic acid derivatives were the most significant compounds in A. trichophylla and C. galaticus, respectively. The highest antioxidant activity of A. trichophylla was mostly exhibited by HAE/methanolic extracts as determined by DPPH, ABTS, FRAP (51.39, 112.70 and 145.73 mg TE/g, respectively) and phosphomolybdenum (2.05 mmol TE/g) assays. However, significant antioxidant activities varied within the extracts of C. galaticus. HAE/methanolic extract of A. trichophylla significantly depressed AChE (2.70 mg GALAE/g), BChE (5.53 mg GALAE/g) and tyrosinase (26.34 mg KAE/g) activities and that of C. galaticus inhibited AChE (2.04 mg GALAE/g), tyrosinase (31.25 mg KAE/g) and α-amylase (0.53 mmol ACAE/g) activities significantly. We concluded that HAE was the most efficient extraction technique as high yield of compounds and promising bioactivities were recorded from extracts prepared. Multivariate analysis showed that types of solvents influenced recovery of compounds and biological activities. This research study can be used as one methodological starting point for further investigation on these plants as all results are clearly promising and open the door to further research challenges such as cytotoxicity evaluation, molecular docking analysis, and more screening of pharmacological actions.

中文翻译：

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来自土耳其的两种药用植物（Akanna trichophila 和 Convolvulus galaticus）：化学特征和生物学观点

本研究的目的是量化选定的酚类化合物，确定Alkanna trichophylla Hub.-Mor地上部分的抗氧化活性和酶抑制作用。( A. trichophylla ) 和Convolvulus galaticus Rost.ex Choisy ( C. galaticus ) 提取物通过均质机辅助提取 (HAE)、浸渍 (MAC) 和灌注技术制备。这是此类研究首次旨在验证这些植物的植物化学成分和生物活性。对收集到的数据进行多变量分析。芦丁和咖啡酰奎宁酸衍生物是A. trichophylla和C. galaticus 中最重要的化合物， 分别。通过 DPPH、ABTS、FRAP（分别为 51.39、112.70 和 145.73 mg TE/g）和磷钼（2.05 mmol TE/g）测定法测定，A. trichophylla的最高抗氧化活性主要由 HAE/甲醇提取物显示。然而，显着的抗氧化活性在C. galaticus的提取物中有所不同。A. trichophylla 的HAE/甲醇提取物显着抑制 AChE (2.70 mg GALAE/g)、BChE (5.53 mg GALAE/g) 和酪氨酸酶 (26.34 mg KAE/g) 以及C. galaticus 的活性显着抑制 AChE (2.04 mg GALAE/g)、酪氨酸酶 (31.25 mg KAE/g) 和 α-淀粉酶 (0.53 mmol ACAE/g) 活性。我们得出的结论是，HAE 是最有效的提取技术，因为从制备的提取物中记录了高产量的化合物和有希望的生物活性。多变量分析表明，溶剂类型会影响化合物的回收率和生物活性。这项研究可以作为对这些植物进行进一步研究的一个方法学起点，因为所有结果显然都有希望，并为进一步研究挑战打开了大门，例如细胞毒性评估、分子对接分析和更多的药理作用筛选。