Abstract

The microporous films comprised of chitosan (CS), gelatin (Gel) and magnesium oxide nanoparticles (MgO NPs) were developed as a wound-healing material using the lyophilization technique. The chemical structure, composition and morphology of the films were evaluated by FTIR, XRD, EDX and SEM. Also, the swelling, mechanical, biodegradation, cell viability, antimicrobial property, bioactivity and cell migration capability of the porous films were determined to evaluate their suitability as a wound-dressing material. The average diameter of pores and porosity of the films were in the range of 73 − 103 μm and 57 − 78%, respectively, based on the content of MgO NPs. Compared to control CS/Gel film, the CS/Gel/MgO films presented improved swelling behavior, mechanical strength, antimicrobial activity against the strains of bacteria and fungi and controlled in vitro degradation. Among the developed films, CS/Gel/MgO films containing 1 wt. % of MgO NPs demonstrated an increased biocompatibility, bioactivity and cell migration ability. These results indicated that the porous films based on CS/Gel/MgO could be a competent dermal substitute for wound healing application.

摘要

使用冻干技术开发了由壳聚糖 (CS)、明胶 (Gel) 和氧化镁纳米粒子 (MgO NPs) 组成的微孔薄膜作为伤口愈合材料。采用FTIR、XRD、EDX和SEM对薄膜的化学结构、组成和形貌进行评价。此外，测定了多孔膜的溶胀、机械、生物降解、细胞活力、抗菌性能、生物活性和细胞迁移能力，以评估其作为伤口敷料材料的适用性。基于 MgO NPs 的含量，薄膜的平均孔隙直径和孔隙率分别在 73 - 103 μm 和 57 - 78% 的范围内。与对照 CS/Gel 薄膜相比，CS/Gel/MgO 薄膜表现出改善的溶胀行为、机械强度、体外降解。在已开发的薄膜中，CS/Gel/MgO 薄膜含有 1 wt. % 的 MgO NPs 表现出增加的生物相容性、生物活性和细胞迁移能力。这些结果表明，基于 CS/Gel/MgO 的多孔薄膜可以成为用于伤口愈合应用的有效皮肤替代品。