Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/μL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex® backing via an ARclean® double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets.

在此，我们描述了一种纸基设备的开发，该设备通过产生人眼可见的比色反应，使用环介导等温扩增 (LAMP) 检测复杂样品中感兴趣的病原体的核酸。为了证明该设备在新出现的突发公共卫生事件中的实用性，我们开发并优化了我们的设备，无需预处理即可检测人类唾液中的 SARS-CoV-2。所得装置能够在 60 分钟内检测到病毒，分析灵敏度为 97%，特异性为 100%，使用图像分析检测限为 200 基因组拷贝/μL 患者唾液。该设备由可配置数量的反应区组成，由 222 级色谱纸构成，由 20 mil 聚苯乙烯垫片隔开，通过 ARclean® 双面粘合剂连接到 Melinex® 背衬。由此产生的设备可轻松配置为检测多个目标，并有可能通过更改 LAMP 引物组来检测各种病原体。