Microbial Pathogenesis ( IF 3.8 ) Pub Date : 2021-08-14 , DOI: 10.1016/j.micpath.2021.105146 Muhammad Zulqarnain Shakir 1 , Farzana Rizvi 1 , M Tariq Javed 1 , M Imran Arshad 2
The present study was conducted to determine the seroprevalence and pathology of Salmonella infection in white commercial layer birds of District Faisalabad during June 2018 and June 2020. The current study aimed to determine the isolation, identification of Salmonella gallinarum (S. gallinarum), its cultural prevalence, antimicrobial resistance, molecular characterization, and pathological lesions produced in different organs of commercial layer birds. Initial screening of poultry flocks was done through serum plate agglutination test followed by culturing in different media, motility test, molecular confirmation, and histopathology. Based on the serum plate agglutination test, seroprevalence in the commercial white layer in dead and live flocks was 40.09%. The cultural prevalence of Salmonella in the seropositive group was 75.36% and in the seronegative was 31.84%. Cultural prevalence in the liver of dead birds was 62.06%, in spleen 58.62%, and in cloacal swabs was 67.24%. A total of 178 isolates were characterized through cultural characteristic and motility tests, among them 63.48% isolates were S. gallinarum, and 36.51% isolates were S. pullorum. The antibiogram study revealed that all the tested isolates were resistant to amoxicillin, gentamycin, kanamycin, doxycyclin, and tetracyclin. While tested isolates were sensitive to ciprofloxacin against S. gallinarum. Pathologically liver was friable, showing bonze discoloration with focal necrosis, enteritis of various grades, mottled white spleen, and enlarged kidneys were found. Microscopically, leukocytic infiltration with focal necrosis and degeneration, in mucosa and submucosa of intestinal inflammatory cells were observed. In conclusion, the seroprevalence, antibiogram, and molecular characterization of Salmonella help to control the disease in a better way through bacterin production of local isolates.
中文翻译:
商品白蛋鸡沙门氏菌感染的血清阳性率和病理学研究
本研究是为了确定的血清阳性率和病理沙门氏菌2018年6月和2020年六月期间感染区费萨拉巴特的白色商业层鸟类目前的研究旨在确定的分离,鉴定沙门氏菌加仑升inarum(鸡沙门氏菌),其文化流行率,抗菌素耐药性,分子特征和在商业蛋鸡不同器官中产生的病理病变。家禽群的初步筛选是通过血清平板凝集试验,然后在不同培养基中培养、运动试验、分子确认和组织病理学进行的。根据血清平板凝集试验,死鸡和活鸡的商品白蛋鸡血清阳性率为 40.09%。血清阳性组沙门氏菌培养率为75.36%,血清阴性组为31.84%。死禽肝脏检出率为62.06%,脾检检出率为58.62%,泄殖腔拭子检出率为67.24%。共对178株分离株进行了培养特征和运动学鉴定,其中63.48%的分离株为S. gallinarum和 36.51% 的分离株是S. pullorum。抗菌谱研究表明,所有受试分离株均对阿莫西林、庆大霉素、卡那霉素、强力霉素和四环素耐药。虽然测试分离株对环丙沙星敏感,但对S. gallinarum敏感。病理上肝脏质脆,呈焦色并有局灶性坏死,有不同程度的肠炎,脾脏呈斑片状白色,肾肿大。镜下观察到肠道炎症细胞黏膜和黏膜下层有白细胞浸润,伴有局灶性坏死和变性。总之,沙门氏菌的血清阳性率、抗菌谱和分子特征 通过生产本地分离株的菌苗,帮助更好地控制疾病。