ABSTRACT

Phosphorus (P) is an important element for plant and bacteria growth. The response of bacteria to P transformation into adsorbed forms and their interaction with plants remain unclear. In this study, the mechanisms involved in P adsorption in plants and bacteria were analyzed by a RNA-seq technology. A phosphate-solubilizing bacterium (PSB) Acinetobacter sp. strain M01 was used in the transcriptome profiling of expressed genes and during interaction with melon (Cucumis melo L.). Results showed that the majority of genes related to cellular component and molecular function were up-regulated. The genes in the melon plant treated with M01 were down-regulated compared with those in the control. Genes for phenylalanine metabolism, amino acid, glycerolipid metabolism were identified in M01 DEGs related to transport and catabolism, carbohydrate metabolisms, and amino acid transport were marked in melon. This study provides an understanding of the gene interaction in PSB to fixed P and melon.

摘要

磷（P）是植物和细菌生长的重要元素。细菌对磷转化为吸附形式的反应及其与植物的相互作用尚不清楚。在这项研究中，通过 RNA-seq 技术分析了植物和细菌中 P 吸附的机制。磷酸盐溶解细菌（PSB）不动杆菌属。菌株 M01 用于表达基因的转录组分析以及与甜瓜（Cucumis meloL.）。结果表明，大多数与细胞成分和分子功能相关的基因上调。与对照中的基因相比，用M01处理的甜瓜植物中的基因被下调。在与运输和分解代谢相关的 M01 DEGs 中鉴定了苯丙氨酸代谢、氨基酸、甘油脂代谢的基因，碳水化合物代谢和氨基酸运输在甜瓜中被标记。该研究提供了对 PSB 与固定 P 和甜瓜的基因相互作用的理解。