Photoacoustic (PA) microscopy (PAM) has achieved remarkable progress in biomedicine in the past decade. It is a fast-rising imaging modality with diverse applications, such as hemodynamics, oncology, metabolism, and neuroimaging. Combining optical excitation and acoustic detection, the hybrid nature of PAM provides advantages of rich contrast and deep penetration. In recent years, high-speed PAM has flourished and enabled high‐speed wide‐field imaging of functional activity. In this review, we summarize the most recent advances in high-speed PAM technologies, including high-repetition-rate multi-wavelength laser development, fast scanning techniques, and novel PA signal acquisition strategies.

中文翻译：

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高速光声显微镜的最新进展

光声（PA）显微镜（PAM）在过去十年中在生物医学领域取得了显着的进展。它是一种快速崛起的成像方式，具有多种应用，例如血流动力学、肿瘤学、新陈代谢和神经成像。PAM 结合光学激发和声学检测，其混合性质提供了丰富的对比度和深度穿透的优势。近年来，高速 PAM 蓬勃发展，实现了功能活动的高速宽视野成像。在这篇综述中，我们总结了高速 PAM 技术的最新进展，包括高重复率多波长激光器开发、快速扫描技术和新颖的 PA 信号采集策略。