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Relationship between elevated microRNAs and growth factors levels in the vitreous of patients with proliferative diabetic retinopathy
Journal of Diabetes and its Complications ( IF 3 ) Pub Date : 2021-08-14 , DOI: 10.1016/j.jdiacomp.2021.108021
Ju Guo 1 , Pengyi Zhou 1 , Meng Pan 1 , Zhenhui Liu 1 , Guangqi An 2 , Jinfeng Han 2 , Fangfang Dai 2 , Liping Du 1 , Xuemin Jin 1
Affiliation  

Aim

The aim of this study was to identify differentially expressed microRNAs (miRNAs) in the vitreous of patients with proliferative diabetic retinopathy (PDR) and correlate some of them with growth factors.

Methods

Vitreous samples were collected from 5 PDR eyes and 5 control eyes, and then miRNAs were assayed with next-generation sequencing (NGS). Three differentially expressed miRNAs were validated in vitreous of another cohort using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR).

Results

Transforming growth factor β (TGF-β) and vascular endothelial growth factor (VEGF) signaling pathway were excavated out through bioinformatic analysis of deregulated miRNAs. The expression of hsa-miR-24-3p, hsa-miR-197-3p and hsa-miR-3184-3p, VEGF-A and TGF-β were confirmed to be significantly higher in the vitreous of PDR eyes than controls(P < 0.05). Furthermore, Pearson's correlation analysis showed significantly positive correlations between these elevated miRNAs and growth factors (P < 0.05).

Conclusions

Elevated vitreous levels of hsa-miR-24-3p, hsa-miR-197-3p, hsa-miR-3184-3p in PDR patients may play roles in pathophysiology of PDR, the target mRNAs of which significantly enriched in VEGF and TGF-β signaling pathways. Positive correlations between elevated vitreous levels of the three miRNAs and VEGF-A, TGF-β in PDR patients could provide a novel research direction for PDR.



中文翻译:

增殖性糖尿病视网膜病变患者玻璃体中升高的 microRNAs 与生长因子水平的关系

目标

本研究的目的是鉴定增殖性糖尿病视网膜病变 (PDR) 患者玻璃体中差异表达的 microRNA (miRNA),并将其中一些与生长因子相关联。

方法

从 5 只 PDR 眼和 5 只对照眼中收集玻璃体样本,然后用下一代测序 (NGS) 检测 miRNA。使用逆转录酶定量聚合酶链反应 (RT-qPCR) 在另一个队列的玻璃体中验证了三个差异表达的 miRNA。

结果

通过对失调的miRNA进行生物信息学分析,挖掘出转化生长因子β(TGF-β)和血管内皮生长因子(VEGF)信号通路。证实 PDR 眼玻璃体中 hsa-miR-24-3p、hsa-miR-197-3p 和 hsa-miR-3184-3p、VEGF-A 和 TGF-β 的表达明显高于对照组(P  < 0.05)。此外,Pearson 相关分析显示,这些升高的 miRNA 与生长因子之间存在显着正相关(P  < 0.05)。

结论

PDR 患者玻璃体中 hsa-miR-24-3p、hsa-miR-197-3p、hsa-miR-3184-3p 水平升高可能在 PDR 的病理生理过程中发挥作用,其靶 mRNA 显着富集 VEGF 和 TGF- β信号通路。PDR患者玻璃体3种miRNA水平升高与VEGF-A、TGF-β呈正相关,可为PDR提供新的研究方向。

更新日期:2021-10-06
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