Purpose

Two myxosporean species have been, so far, independently reported from the gallbladder of the European pilchard, Sardina pilchardus (Walbaum) (synonym Clupea pilchardus) in the Northern shore of the Mediterranean Sea; Ceratomyxa truncata Thélohan, and Coccomyxa morovi Léger and Hesse, 1907. The two species were described with incomplete morphological data and based only on line drawings of their mature myxospores.

Methods

During a parasitological survey in the Southern shores of the Mediterranean coast in the gulf of Gabès off Tunisia, two coelozoic myxosporean species were found in the European pilchard and described using morphological and molecular phylogenetic tools. Morphological characterization was based on the mature myxospore study and some vegetative stages. The SSU rDNA sequences were performed for molecular and phylogenetic study.

Results

The most frequently encountered species belongs to the genus Ceratomyxa Thelohan, 1892. The second species belongs to the genus Coccomyxa. Morphological examinations, allowed us to match these two recorded species with Ceratomyxa truncata and Coccomyxa morovi, respectively, as previously described in the same host species referring to the original manuscripts instead of some morphological differences. Molecular analyses based on the partial SSU rDNA sequences did not much with any of the previously reported myxozoan sequences. Phylogenetic analysis positioned C. truncate in a well-supported clade including Ceratomyxa ssp. from Mediterranean Sea, while C. morovi was positioned on the basis of the subclade grouping all Coccomyxidae species.

Conclusion

We provided herein a first morphological redescription of Ceratomyxa truncata and Coccomyxa morovi parasite of Sardina pilchardus from the Southern shores of the Mediterranean Sea and we successfully obtained the SSU rDNA sequences of these two species and positioned them in the phylogenetic tree.

中文翻译：

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Ceratomyxa truncata Thelohan (1895) 和 Cocccomyxa morovi Léger and Hesse, 1907 (Myxozoa: Myxosporea) 感染突尼斯海岸沙丁鱼 (Walbaum) 胆囊的形态学重新描述和系统发育位置

目的

迄今为止，从欧洲沙丁鱼的胆囊中独立报道了两种粘孢子虫，位于地中海北岸的沙丁鱼沙丁鱼（Walbaum）（同义词Clupea pilchardus ）；Ceratomyxa truncata Thélohan 和Coccomyxa morovi Léger 和 Hesse，1907 年。这两个物种的形态学数据不完整，仅基于其成熟粘孢子的线条图。

方法

在突尼斯附近加贝斯湾地中海沿岸南岸的寄生虫学调查中，在欧洲沙丁鱼中发现了两种腔生代粘孢子物种，并使用形态学和分子系统发育工具进行了描述。形态特征是基于成熟的粘孢子研究和一些营养阶段。SSU rDNA 序列用于分子和系统发育研究。

结果

最常见的物种属于Ceratomyxa Thelohan 属，1892 年。第二个物种属于Coccomyxa属。形态学检查使我们能够将这两个记录的物种分别与Ceratomyxa truncata和Coccomyxa morovi进行匹配，如先前在相同宿主物种中所描述的，指的是原始手稿而不是一些形态差异。基于部分 SSU rDNA 序列的分子分析与任何先前报道的粘虫序列没有太大关系。系统发育分析将C. truncate定位在一个得到良好支持的进化枝中，包括Ceratomyxa ssp。来自地中海，而C. morovi被定位在所有 Cocomyxidae 物种的亚进化枝的基础上。

结论

我们在此提供了对来自地中海南岸的沙丁鱼的Ceratomyxa truncata和Coccomyxa morovi寄生虫的第一次形态学重新描述，我们成功地获得了这两个物种的 SSU rDNA 序列并将它们定位在系统发育树中。