Our research targets on the quantitative determination of protein-to-ligand binding in complex cellular environments. Fluorine protein labelling has been utilized enabling to analyze signal height, line width and chemical shift upon DNA binding in great detail employing high-resolution NMR spectroscopy and simulations. This approach can be easily applied to studies generally interested in interaction processes taking place in cellular environments.

中文翻译：

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氟核磁共振波谱能够量化细胞裂解物中 DNA 和蛋白质之间的亲和力

我们的研究目标是定量测定复杂细胞环境中蛋白质与配体的结合。氟蛋白标记已被用来利用高分辨率 NMR 光谱和模拟来详细分析 DNA 结合时的信号高度、线宽和化学位移。这种方法可以很容易地应用于通常对细胞环境中发生的相互作用过程感兴趣的研究。