Heterologous expression of eukaryotic gene clusters in yeast has been widely used for producing high-value chemicals and bioactive secondary metabolites. However, eukaryotic transcription cis-elements are still undercharacterized, and the cross-species expression mechanism remains poorly understood. Here we used the whole expression unit (including original promoter, terminator, and open reading frame with introns) of orotidine 5′-monophosphate decarboxylases from 14 Penicillium species as a showcase, and analyzed their cross-species expression in Saccharomyces cerevisiae. We found that pyrG promoters from the Penicillium species could drive URA3 expression in yeast, and that inefficient cross-species splicing of Penicillium introns might result in weak cross-species expression. Thus, this study demonstrates cross-species expression from Penicillium to yeast, and sheds light on the opportunities and challenges of cross-species expression of fungi expression units and gene clusters in yeast without refactoring for novel natural product discovery.

中文翻译：

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从青霉到酿酒酵母的跨物种转录和剪接的表征

真核基因簇在酵母中的异源表达已被广泛用于生产高价值化学品和具有生物活性的次级代谢产物。然而，真核转录顺式元件的特征仍然不足，跨物种表达机制仍然知之甚少。在这里，我们使用来自 14 个青霉属物种的乳清酸 5'-单磷酸脱羧酶的整个表达单元（包括原始启动子、终止子和带有内含子的开放阅读框）作为展示，并分析了它们在酿酒酵母中的跨物种表达。我们发现来自青霉属物种的pyrG启动子可以驱动酵母中URA3的表达，并且青霉属内含子的低效跨物种剪接可能导致弱的跨物种表达。因此，