In this work formation of silver nanoparticles using pomegranate fruit (Punica granatum) extract and its bio applications were studied. Gradual appearance of a peak at 422 nm during the reduction of AgNO₃ by the fruit extract confirmed the formation of AgNPs. FTIR spectra indicated the details of formation mechanism. Images obtained from scanning electron microscopy (SEM) and transmittance electron microscopy (TEM) confirmed that the sample contains spherical AgNPs and XRD analysis showed its (FCC) crystal structure. This study also revealed that the synthesised AgNPs were monocrystalline in nature. Application of these AgNPs were studied measuring their affinity to bovine serum albumin (BSA) and human serum albumin (HSA). Cytotoxicity of the synthesised AgNPs was determined by DAPI staining. The results showed that the nanoparticles have significant anticancer activity against triple negative breast carcinoma and MDA-MB-468 cells. We concluded that the cell death was routed through apoptotic pathway.

在这项工作中，研究了使用石榴果实 ( Punica granatum ) 提取物形成银纳米粒子及其生物应用。在 AgNO ₃还原过程中，422 nm 处的峰逐渐出现通过水果提取物证实了 AgNPs 的形成。FTIR 光谱显示了形成机制的细节。从扫描电子显微镜 (SEM) 和透射电子显微镜 (TEM) 获得的图像证实样品含有球形 AgNPs，XRD 分析显示其 (FCC) 晶体结构。该研究还表明，合成的 AgNPs 本质上是单晶的。研究了这些 AgNPs 的应用，测量它们对牛血清白蛋白 (BSA) 和人血清白蛋白 (HSA) 的亲和力。合成的 AgNPs 的细胞毒性由 DAPI 染色确定。结果表明，纳米颗粒对三阴性乳腺癌和MDA-MB-468细胞具有显着的抗癌活性。我们得出结论，细胞死亡是通过细胞凋亡途径进行的。