Abstract

By applying a mixed-ligand approach, two new Cu(II)-containing coordination polymers (CPs) with the chemical formula of [Cu(L)(OBA)(H₂O)]_n (1, L = 1,4-bis(5,6-dimethylbenzimidazol-1-yl)-2-butylene; H₂OBA = 4,4′-oxybis(benzoic acid)) and {[Cu₂(L)₂(1,4-NDC)₂(H₂O)]·8.5H₂O}_n (2) (1,4-H₂NDC = 1,4-naphthalenedicarboxylic acid) were solvothermally synthesized. Their protective activity against the nonalcoholic fatty liver disease (NAFLD) was evaluated and the detail mechanism was detected. Firstly, the Triglyceride detection kit was used to measure the content of the Triglyceride in the plasma after compounds 1 and 2 treatments. Besides, the real time RT-PCR was performed to detect the relative expression level of the expression level of the inflammatory genes nf-κb and p53 in the liver cells.

摘要

通过应用混合配体方法，两种新的含 Cu(II) 的配位聚合物 (CP) 的化学式为 [Cu(L)(OBA)(H ₂ O)] _n ( 1 , L = 1,4-双(5,6-二甲基苯并咪唑-1-基)-2-丁烯；H ₂ OBA = 4,4'-氧基双(苯甲酸))和{[Cu ₂ (L) ₂ (1,4-NDC) ₂ ( H ₂ O)]·8.5H ₂ O} _n ( 2 ) (1,4-H ₂NDC = 1,4-萘二甲酸）是溶剂热合成的。评估了它们对非酒精性脂肪性肝病 (NAFLD) 的保护活性，并检测了详细机制。首先用甘油三酯检测试剂盒测定化合物1和2处理后血浆中甘油三酯的含量。此外，进行实时RT-PCR以检测炎症基因nf-κb和p53在肝细胞中表达水平的相对表达水平。