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β-HB inhibits the apoptosis of high glucose-treated astrocytes via activation of CREB/BDNF axis
Brain Injury ( IF 1.9 ) Pub Date : 2021-08-12 , DOI: 10.1080/02699052.2021.1959061
Lei Zhang 1 , Da-Long Dong 2 , Jun-Hong Gao 3 , An-Kui Wang 2 , Yong-Ping Shao 1
Affiliation  

ABSTRACT

Objective

Nerve damage can cause severe limb dysfunction and even leave a lifelong disability. The apoptosis of astrocytes may contribute to the nerve damage. In this research, we sought to investigate the effect of β-HB on nerve damage in vitro.

Design

Astrocytes were treated with high glucose (HG) to mimic in vitro model of nerve damage. RT-qPCR and western blot were used to detect expressions of CREB, BDNF, Ki-67, PCNA, Bax, Bcl-2 and cleaved caspase 3 in astrocytes, respectively. MTT was used to measure the cell viability. In addition, flow cytometry was used to detect the cell apoptosis.

Results

β-HB significantly promoted the proliferation and inhibited apoptosis in HG-treated astrocytes. Results showed that of PCNA and Bcl-2 were upregulated, and Bax and cleaved caspase 3 were downregulated after β-HB stimulated in HG-treated astrocytes. In addition, HG-induced inhibition on BDNF expression in astrocytes was notably reversed by β-HB. Furthermore, β-HB promoted the growth and inhibited apoptosis of high glucose-treated astrocytes via activation of CREB/BDNF axis.

Conclusion

β-HB promotes the growth and inhibits the apoptosis of high glucose-treated astrocytes via activation of CREB/BDNF axis, which may serve as a new target for treatment of nerve damage.



中文翻译:

β-HB通过激活CREB/BDNF轴抑制高糖处理的星形胶质细胞凋亡

摘要

客观的

神经损伤可导致严重的肢体功能障碍,甚至终身残疾。星形胶质细胞的凋亡可能导致神经损伤。在这项研究中,我们试图研究 β-HB 对体外神经损伤的影响

设计

星形胶质细胞用高糖 (HG) 处理以模拟神经损伤的体外模型。RT-qPCR和蛋白质印迹分别用于检测星形胶质细胞中CREB、BDNF、Ki-67、PCNA、Bax、Bcl-2和cleaved caspase 3的表达。MTT用于测量细胞活力。此外,流式细胞术用于检测细胞凋亡。

结果

β-HB 显着促进 HG 处理的星形胶质细胞的增殖并抑制其凋亡。结果表明,在 HG 处理的星形胶质细胞中,β-HB 刺激后,PCNA 和 Bcl-2 上调,Bax 和裂解的 caspase 3 下调。此外,β-HB 显着逆转了 HG 对星形胶质细胞中 BDNF 表达的抑制。此外,β-HB 通过激活 CREB/BDNF 轴促进高糖处理星形胶质细胞的生长并抑制其凋亡。

结论

β-HB通过激活CREB/BDNF轴促进高糖处理星形胶质细胞的生长并抑制其凋亡,这可能成为治疗神经损伤的新靶点。

更新日期:2021-10-01
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