Introduction

Bromelain is a complex mixture of thiol proteases and other non-proteolytic constituents, commercially extracted primarily from the pineapple stem. Evidence from several in vitro and in vivo studies highlights its excellent bioavailability, lack of side effects, and broad spectrum of medical efficacies, of which the antiphlogistic properties are among the most valuable ones. Bromelain has indeed been employed for the efficient treatment of many inflammatory disorders, ranging from osteoarthritis and inflammatory bowel diseases to cancer-related inflammation.

Methods

The aim of the current study was to assess the anti-inflammatory effects of bromelain after gastrointestinal digestion simulated in vitro using stomach, intestinal, and chondrocyte human cellular models (AGS, Caco-2, and SW1353, respectively).

Results

We successfully demonstrated the capability of bromelain to reduce an inflammatory stimulus by reproducing its exposure to the gastro-enteric environment in vitro and assaying its effect in human cell lines derived from stomach, intestinal, and chondrocytes.

Conclusion

Consistently with the previously published data, our work underpins the relevance of bromelain in the development of safer and more effective anti-inflammatory therapies.

中文翻译：

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在体外模拟胃肠消化后，专有菠萝蛋白酶提取物 (Bromeyal™) 的抗炎特性

介绍

菠萝蛋白酶是硫醇蛋白酶和其他非蛋白水解成分的复杂混合物，商业上主要从菠萝茎中提取。多项体外和体内研究的证据表明其具有优异的生物利用度、无副作用和广谱的医疗功效，其中抗炎特性是最有价值的特性之一。菠萝蛋白酶确实已被用于有效治疗许多炎症性疾病，从骨关节炎和炎症性肠病到癌症相关炎症。

方法

本研究的目的是使用胃、肠​​和软骨细胞人类细胞模型（分别为 AGS、Caco-2 和 SW1353）在体外模拟胃肠消化后评估菠萝蛋白酶的抗炎作用。

结果

我们通过在体外复制其对胃肠环境的暴露并测定其在源自胃、肠和软骨细胞的人类细胞系中的作用，成功地证明了菠萝蛋白酶减少炎症刺激的能力。

结论

与之前发表的数据一致，我们的工作支持菠萝蛋白酶在开发更安全、更有效的抗炎疗法中的相关性。